A simple and highly sensitive method is developed for the determination of streptomycin in dosage forms and in biological fluids using ion exchange-high-performance thin-layer chromatography (IE-HPTLC). The method involves the use of titanium(IV) silicate (a synthetic inorganic ion exchanger) coated plates and a mixture of KBr (0.5 m) and ethanol (9:1, ν/ν) as mobile phase. The development time was 18 min. Quantitative measurements were made using a CAMAG TLC Scanner-3 at 359 nm. The streptomycin recovery from the total procedure was equal to 97.9%. The procedure is quantitatively characterized, the linearity was r2 > 0.99, and the relative standard deviation (RSD) was <0.94. The limit of detection (LOD) and limit of quantification (LOQ) were 2.4 and 12 ng, respectively.
M.A. Sande, G.L. Mandell, in: L.S. Goodman, A.G. Gilman, T.W. Rail, F. Murad (eds) The Pharmacological Basis of Therapeutics, 7th edn., MacMillan, New York, 1985, p. 1150.
Mandell G.L., '', in The Pharmacological Basis of Therapeutics, 7th edn., (1985) -.
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