This paper presents the development and validation of an improved method for the analysis of fluconazole using high-performance thinlayer chromatography (HPTLC) with densitometric detection. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of ethyl acetate-methanol-ammonia-diaminoethane (85:10:5:0.5 ν/ν/ν/ν). Detection wavelength was 216 nm and the RF value obtained was 0.4 ± 0.03. An F-test indicated that the calibration graph was linear at the evaluated concentration range. The %RSD for repeatability and intermediate precision were 1.60 and 3.05, respectively. The mean percentage recovery for the trueness was 99.1 ± 2.1%. This method was successfully used to analyze the fluconazole content in marketed tablet samples.
Guidelines for Prevention and Treatment of Opportunistic Infections in HIV-Infected Adults and Adolescents, Morbidity and Mortality Weekly Report, Volume 58, 2009,
; accessed on 14 March 2010
P.G. Risha, Z. Msuya, M. Clark, K. Johnson, M.N. Sigonda, T. Layloff, Health Policy 87 (2008) 217–222.
Layloff T., '' (2008) 87Health Policy: 217-222.
Layloff T.Health Policy200887217222)| false
D.H. Shewiyo, E. Kaale, P.G. Risha, B. Dejaegher, J. Smeyers-Verbeke, Y. Vander Heyden, J. Chromatogr. A 1216 (2009) 7102–7107.
Guidelines prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human use (ICH), Validation of Analytical Procedure: Text and Methodology, Q2(R1), 2005, 1–13,
; accessed on 24 July 2009
J.C. Touchstone, Practice of Thin Layer Chromatography, 3rd edn., John Wiley & Sons, New York, 1992.
Touchstone J.C., '', in Practice of Thin Layer Chromatography, (1992) -.
Touchstone J.C.Practice of Thin Layer Chromatography1992)| false
D.L. Massart, B.G.M. Vandeginste, L.M.C. Buydens, S. De Jong, P.J. Lewi, J. Smeyers-Verbeke, Handbook of Chemometrics and Qualimetrics: Part A, Elsevier, Amsterdam, 1997.
Smeyers-Verbeke J., '', in Handbook of Chemometrics and Qualimetrics: Part A, (1997) -.
Smeyers-Verbeke J.Handbook of Chemometrics and Qualimetrics: Part A1997)| false
D.H. Shewiyo, E. Kaale, C. Ugullum, M.N. Sigonda, P.G. Risha, B. Dejaegher, J. Smeyers-Verbeke, Y. Vander Heyden, J. Pharm. Biomed. Anal. 54 (2011) 445–450.