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  • 1 Tanzania Food and Drugs Authority P. O. Box 77150 Dar es Salaam Tanzania
  • | 2 Muhimbili University of Health and Allied Sciences P. O. Box 65013 Dar es Salaam Tanzania
  • | 3 Tanzania Food and Drugs Authority P. O. Box 77150 Dar es Salaam Tanzania
  • | 4 Vrije Universiteit Brussel Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research Laarbeeklaan 103 1090 Brussels Belgium
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This paper presents the development and validation of an improved method for the analysis of fluconazole using high-performance thinlayer chromatography (HPTLC) with densitometric detection. Separation was performed on silica gel 60 F254 plates. The mobile phase is comprised of ethyl acetate-methanol-ammonia-diaminoethane (85:10:5:0.5 ν/ν/ν/ν). Detection wavelength was 216 nm and the RF value obtained was 0.4 ± 0.03. An F-test indicated that the calibration graph was linear at the evaluated concentration range. The %RSD for repeatability and intermediate precision were 1.60 and 3.05, respectively. The mean percentage recovery for the trueness was 99.1 ± 2.1%. This method was successfully used to analyze the fluconazole content in marketed tablet samples.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
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Address
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Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
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Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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