JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 6
A simple, rapid, and sensitive HPTLC method for the estimation of clarithromycin: Application to single dose clinical study
Authors:
Punnamchand Loya Principal K.M. Kundnani College of Pharmacy Plot No. 23, Joy Building, Rambhau, Salgaonkar Marg, Cuffe parade Colaba, Mumbai 400 005 India

Search for other papers by Punnamchand Loya in
Current site
Google Scholar
PubMed Close
and
Poornima Hamrapurkar Principal K.M. Kundnani College of Pharmacy Plot No. 23, Joy Building, Rambhau, Salgaonkar Marg, Cuffe parade Colaba, Mumbai 400 005 India

Search for other papers by Poornima Hamrapurkar in
Current site
Google Scholar
PubMed Close
Pages:
534–538
Online Publication Date:
02 Dec 2011
Publication Date:
01 Dec 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.6.15
Keywords:
Clarithromycin; Human plasma; High-performance thin-layer chromatography (HPTLC); Application
Restricted access

Clarithromycin used for the treatment of respiratory tract infection. Anew alternative simple, rapid, sensitive, and selective high-performance thin-layer chromatography (HPTLC) method was developed and validated for the estimation of clarithromycin in plasma. HPTLC analysis was performed on pre-coated silica gel 60F254 plates. Separation was carried out using ethyl acetate-methanol-15% ammonium acetate (pH 10.6) as solvent system. Densitometry measurements were performed in absorbance mode at 506 nm after derivatising with xanthydrol solution. The RF of the drug was found to be 0.62. The method was linear over the range of 0.1–3.0 μg mL−1 (r2 = 0.9974). The mean extraction recoveries were over 85%. The intra-day and inter-day precision (%CV) of the assay was in the range of 0.79–4.55%. The accuracy was found to be above 95%. The method was successfully applied to a pharmacokinetic study of clarithromycin 250 mg tablet in healthy human male volunteers.

  • S.C. Sweetman (ed.) Martindale: The Complete Drug Reference, Pharmaceutical Press, London, 34th edn., 2005, 192–193.

    '', in Martindale: The Complete Drug Reference , (2005 ) -.

  • D.K. Morgan, D.M. Brown, T.D. Rotsch, A.C. Plasz, J. Pharm. Biomed. Anal. 9 (1991) 261–269.

    Plasz A.C. , '' (1991 ) 9 J. Pharm. Biomed. Anal. : 261 -269 .

  • D. Morgan, P. Cugier, B. Marello, C. Sarocka, D. Stroz, A. Plasz, J. Chromatogr. A 502 (1990) 351–358.

    Plasz A. , '' (1990 ) A502 J. Chromatogr. : 351 -358 .

  • P.O. Erah, D.A. Barrett, P.N. Shaw, J. Chromatogr. B 682 (1996) 73–78.

    Shaw P.N. , '' (1996 ) B682 J. Chromatogr. : 73 -78 .

  • J.S. Torano, H.J. Guchelaar, J. Chromatogr. B 720 (1998) 89–97.

    Guchelaar H.J. , '' (1998 ) B720 J. Chromatogr. : 89 -97 .

  • G. Bahrami, B. Mohammad, J. Chromatogr. B 850 (2007) 417–422.

    Mohammad B. , '' (2007 ) B850 J. Chromatogr. : 417 -422 .

  • J.I.D. Wibawa, P.N. Shaw, D.A. Barrett, J. Chromatogr. B 783 (2003) 359–366.

    Barrett D.A. , '' (2003 ) B783 J. Chromatogr. : 359 -366 .

  • A.P. Louisi, A. Papageorgiou, A. Zitrou, S. Sotiropoulos, E. Georgarakis, F. Zougrou, J. Chromatogr. B 755 (2001) 57–64.

    Zougrou F. , '' (2001 ) B755 J. Chromatogr. : 57 -64 .

  • C. Taninaka, H. Ohtani, E. Hanada, H. Kotaki, H. Sato, T. Iga, J. Chromatogr. B 738 (2000) 405–411.

    Iga T. , '' (2000 ) B738 J. Chromatogr. : 405 -411 .

  • I. Niopas, A.C. Daftsios, Biomed. Chromatogr. 15 (2001) 507–508.

    Daftsios A.C. , '' (2001 ) 15 Biomed. Chromatogr. : 507 -508 .

  • S.Y. Chu, L.T. Sennello, R.C. Sonders, J. Chromatogr. B 571 (1991) 199–208.

    Sonders R.C. , '' (1991 ) B571 J. Chromatogr. : 199 -208 .

  • A.K. Lee, J.H. Lee, J.W. Kwon, W.B. Kim, S.G. Kim, S.H. Kim, M.G. Lee, Biopharm. Drug Dispos. 25 (2004) 273–282.

    Lee M.G. , '' (2004 ) 25 Biopharm. Drug Dispos. : 273 -282 .

  • O. Lohitnavy, M. Lohitnavy, K. Sareekan, S. Polnok, P. Taytiwa, Biopharm. Drug Dispos. 24 (2003) 229–231.

    Taytiwa P. , '' (2003 ) 24 Biopharm. Drug Dispos. : 229 -231 .

  • W. Li, J. Rettig, X. Jiang, D.T. Francisco, W. Naidong, Biomed. Chromatogr. 20 (2006) 1242–1251.

    Naidong W. , '' (2006 ) 20 Biomed. Chromatogr. : 1242 -1251 .

  • G.F. Van Rooyen, M.J. Smit, A.D. de Jager, H.K.L. Hundt, K.J. Swart, A.F. Hundt, J. Chromatogr. B 768 (2002) 223–229.

    Hundt A.F. , '' (2002 ) B768 J. Chromatogr. : 223 -229 .

  • Y. Jiang, J. Wang, H. Li, Y. Wang, J. Gu, J Pharm. Biomed. Anal. 43 (2007) 1460–1464.

    Gu J. , '' (2007 ) 43 J Pharm. Biomed. Anal. : 1460 -1464 .

  • H. Vanderhaeghe, J. Pharm. Biomed. Anal. 7 (1989) 127–128.

    Vanderhaeghe H. , '' (1989 ) 7 J. Pharm. Biomed. Anal. : 127 -128 .
  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 64 0 0
Dec 2024 46 0 0
Jan 2025 19 0 0
Feb 2025 21 0 0
Mar 2025 33 0 0
Apr 2025 13 0 0
May 2025 0 0 0