Clarithromycin used for the treatment of respiratory tract infection. Anew alternative simple, rapid, sensitive, and selective high-performance thin-layer chromatography (HPTLC) method was developed and validated for the estimation of clarithromycin in plasma. HPTLC analysis was performed on pre-coated silica gel 60F254 plates. Separation was carried out using ethyl acetate-methanol-15% ammonium acetate (pH 10.6) as solvent system. Densitometry measurements were performed in absorbance mode at 506 nm after derivatising with xanthydrol solution. The RF of the drug was found to be 0.62. The method was linear over the range of 0.1–3.0 μg mL−1 (r2 = 0.9974). The mean extraction recoveries were over 85%. The intra-day and inter-day precision (%CV) of the assay was in the range of 0.79–4.55%. The accuracy was found to be above 95%. The method was successfully applied to a pharmacokinetic study of clarithromycin 250 mg tablet in healthy human male volunteers.
S.C. Sweetman (ed.) Martindale: The Complete Drug Reference, Pharmaceutical Press, London, 34th edn., 2005, 192–193.
'', in Martindale: The Complete Drug Reference, (2005) -.
Martindale: The Complete Drug Reference2005)| false
D.K. Morgan, D.M. Brown, T.D. Rotsch, A.C. Plasz, J. Pharm. Biomed. Anal. 9 (1991) 261–269.