A simple high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous quantification of penta-O-galloyl-β-d-glucose (PGG), tetra-O-galloyl-β-d-glucose (TGG), chebulinic acid, chebulagic acid, and gallic acid from different samples of Triphala churna: TC1, TC2, TC3, TC4, Hirda, Behda, Amla. The method was validated for precision (expressed as coefficient of variation, CV [%]), accuracy, sensitivity, and selectivity. The accuracy of the method was determined by measurement of the recovery at three different concentrations. Average recovery was 98.46% for PGG, 100.02% for TGG, 100.05% for chebulinic acid, 99.79% for chebulagic acid, and 101.15% for gallic acid from Triphala churna. The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of the different samples of Triphala churna containing hydrolyzable tannins.
P.H. Kulkarni, Clinical Assessment of the Effect of Sookshma Triphala in Lipoma, Ayurvedic Research Papers II, Pune, 1995, pp. 66–71.
Kulkarni P.H., '', in Clinical Assessment of the Effect of Sookshma Triphala in Lipoma, (1995) -.
Kulkarni P.H.Clinical Assessment of the Effect of Sookshma Triphala in Lipoma1995)| false