View More View Less
  • 1 IPS Academy College of Pharmacy Indore 452015 Madhya Pradesh India
Restricted access

A stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been developed for the determination of simultaneous determination of famotidine (FAMO), paracetamol (PCM), and ibuprofen (IBU) in tablet dosage forms. The separation was achieved on TLC aluminum plates precoated with silica gel 60F-254 using chloroform-methanol-ethyl acetate-acetic acid (21.5:16.1:59.1:3.2%, v/v/v/v) as the mobile phase. The densitometric analysis was carried out at 256 nm. Compact spots appeared at RF 0.21 ± 0.01, 0.80 ± 0.02, and 0.89 ± 0.01 for FAMO, PCM, and IBU, respectively. Linear regression analysis revealed linearity in the range of 160–960 ng per spot for FAMO, 400–2400 ng per spot for PCM, and 600–3600 ng per spot for IBU. Drugs were subjected to acid and alkali hydrolyses, forced oxidation, thermal and photodegradation treatments. The degraded products were well separated from the pure drugs. Statistical analysis proved that the method is precise, accurate, selective, and economical and may be used for routine analysis of FAMO, PCM, and IBU in tablet dosage forms.

  • P.D. Sethi, HPTLC High Performance Thin Layer Chromatography, 1st edn., CBS Publishers, New Delhi, 1996, pp. 1–68.

    Sethi P.D. , '', in HPTLC High Performance Thin Layer Chromatography , (1996 ) -.

  • H.H. Willard, L.L. Merritt, J.A. Dean, F.A. Settle, Instrumental Methods of Analysis, 7th edn., CBS Publishers, New Delhi, 1986, pp. 513–534.

    Settle F.A. , '', in Instrumental Methods of Analysis , (1986 ) -.

  • J.E.F. Reynolds, K. Parfitt, A.V. Partons, S.C. Sweetman, The Extra Pharmacopoeia, Martindale, 31st edn., Pharmaceutical Press, London, 1996, pp. 50–51, 81–82, 1218–1223.

    Sweetman S.C. , '', in The Extra Pharmacopoeia, Martindale , (1996 ) -.

  • S.M. Pawar, B.S. Patil, R.Y. Patil, Inter. J. Adv. Pharm. Sci. 1 (2010) 54–59.

    Patil R.Y. , '' (2010 ) 1 Inter. J. Adv. Pharm. Sci. : 54 -59.

  • J. Novakovic, J. Chromatogr. A 846 (1999) 193–198.

    Novakovic J. , '' (1999 ) 846 J. Chromatogr. A : 193 -198.

  • A. Yadav, R.M. Singh, S.C. Mathur, P.K. Saini, G.N. Singh, J. Planar Chromatogr. 22(6) (2009) 421–424.

    Singh G.N. , '' (2009 ) 22 J. Planar Chromatogr. : 421 -424.

  • A. Thenmozhi, D. Sridharan, J.F.M. Titus, M. Palanivelu, J. Pharm. Recent Res. 1 (2009) 22–26.

    Palanivelu M. , '' (2009 ) 1 J. Pharm. Recent Res. : 22 -26.

  • F. Soponar, A.C. Mot, C. Sarbu, Chromatographia 69 (2009) 151–155.

    Sarbu C. , '' (2009 ) 69 Chromatographia : 151 -155.

  • V.K. Mahajan, S.B. Bari, A.A. Shirkhedkar, S.J. Surana, Acta Chromatogr. 20 (2008) 625–636.

    Surana S.J. , '' (2008 ) 20 Acta Chromatogr. : 625 -636.

  • T.K. Save, D.V. Parmar, P.V. Devarajan, J. Chromatogr. B 69 (1997) 315–319.

    Devarajan P.V. , '' (1997 ) 69 J. Chromatogr. B : 315 -319.

  • S.S. Chitlange, D.M. Sakarkar, S.B. Wankhede, S.G. Wadodkar, Indian J. Pharm. Sci. 70 (2008) 398–400.

    Wadodkar S.G. , '' (2008 ) 70 Indian J. Pharm. Sci. : 398 -400.

  • N. Dubey, N. Dubey, R.S. Mehta, A.K. Saluja, J. AOAC Int. 92 (2009) 1617–1621.

    Saluja A.K. , '' (2009 ) 92 J. AOAC Int. : 1617 -1621.

  • N. Dubey, N. Dubey, R.S. Mehta, A.K. Saluja, J. AOAC Int. 92 (2009) 1021–1026.

    Saluja A.K. , '' (2009 ) 92 J. AOAC Int. : 1021 -1026.

  • N. Dubey, N. Dubey, R.S. Mehta, A.K. Saluja, J. AOAC Int. 92 (2009) 779–784.

    Saluja A.K. , '' (2009 ) 92 J. AOAC Int. : 779 -784.

  • K. Ferenczi-Fodor, B. Renger, Z. Végh, J. Planar Chromatogr. 23 (2010) 173–179.

    Végh Z. , '' (2010 ) 23 J. Planar Chromatogr. : 173 -179.

  • A.A. Shirkhedkar, R.P. Thorve, R.A. Fursule, S.J. Surana, Acta Chromatogr. 20 (2008) 423–437.

    Surana S.J. , '' (2008 ) 20 Acta Chromatogr. : 423 -437.

All Time Past Year Past 30 Days
Abstract Views 85 84 13
Full Text Views 14 11 0
PDF Downloads 13 13 0