JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 25: Issue 2
Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of arbutin in commercial whitening creams
Authors:
Saleh Alqasoumi

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Prawez Alam Salman Bin AbdulAziz University Department of Pharmacognosy, College of Pharmacy P.O. Box 173 Al-Kharj 11942 Kingdom of Saudi Arabia

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Maged Abdel-Kader Salman Bin AbdulAziz University Department of Pharmacognosy, College of Pharmacy P.O. Box 173 Al-Kharj 11942 Kingdom of Saudi Arabia

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Pages:
168–173
Online Publication Date:
29 Mar 2012
Publication Date:
01 Apr 2012
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.25.2012.2.14
Keywords:
Arbutin; TLC densitometry; ICH guidelines; Qualitative; Quantitative; Whitening creams
Restricted access

High-performance thin-layer chromatographic (HPTLC) densitometric method for analysis of arbutin in commercial whitening creams was developed and validated. Aluminum-backed silica gel 60 F254 plates were used as stationary phase while methanol-chloroform-acetic acid 3.5:6:0.5 (%, v/v/v) mixture was used as mobile phase. Under these chromatographic conditions, arbutin was well separated from other ingredients. This system was found to give a well defined, sharp, and compact spot of arbutin at retention factor (RF) value of 0.40 ± 0.02. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 42.25 and 112.45 ng per spot respectively. The proposed method with high degree of precision and accuracy was employed for the analysis of arbutin both qualitatively and quantitatively in commercial whitening creams. Due to the efficiency of the method in separating arbutin from other ingredients including its degradation products, it can be applied as a stability-indicating method.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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