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  • 1 University of Hohenheim Institute of Food Chemistry Garbenstrasse 28 70599 Stuttgart Germany
  • 2 Justus-Liebig-University of Gießen Institute of Nutritional Science Heinrich-Buff-Ring 26 35392 Gießen Germany
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High-performance thin-layer chromatography (HPTLC) proved to be a well-suited method for characterization and assignment of polysaccharide-based biopolymers in all types of plant-derived or modified thickening agents, stabilizers, or hydrocolloids on the market. The newly developed HPTLC method allows for differentiation of the polysaccharides due to its characteristic fingerprint. It is a highly effective, rapid, and cost-saving alternative or complementary method to current official methods. After a reduced sample preparation including methanolysis of the polysaccharides, up to 21 samples were separated on HPTLC silica gel plates simultaneously under identical conditions in 20 min. For determination of the methylated monomeric units in the UV-VIS range, a selective postchromatographic derivatization was performed using the aniline diphenylamine o-phosphoric acid reagent. Via spectra recording, the maximum wavelengths were obtained and absorption measurement was performed at various wavelengths showing the potential for quantification based on selected marker compounds. The limit of detection (LOD) of the methylated monomeric units was by a factor of 5 worse if compared to the non-methylated monomeric units, but this was not critical for analysis.

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