The purpose of this study was to identify and quantify various neutral and polar lipids in certain organs of mice using high-performance thin-layer chromatography (HPTLC). Four mice infected with Schistosoma mansoni and three control mice were used for this study. At 6 weeks postinfection, the mice were necropsied, and the liver, spleen, and small intestine were removed and prepared for lipid analysis. Lipids were separated on laned, preadsorbant Analtech HPTLC-HLF 20 × 10-cm silica gel plates. Neutral lipids were separated using petroleum ether-diethyl ether-glacial acetic acid (80:20:1) mobile phase and were detected by spraying with 5% ethanolic phosphomolybdic acid detection reagent. Polar lipids were separated with chloroform-methanol-deionized water (65:25:4) mobile phase and detected using 10% cupric sulfate in 8% phosphoric acid reagent. The analyzed neutral lipids were free sterols, free fatty acids, and triacylglycerols. Using HPTLC, no significant differences were found in these lipids between the infected and uninfected mice organs. The polar lipids analyzed were phosphatidylcholine (PC) and phospatidylethanolamine (PE). There was a significantly higher PC content in the liver and small intestine of the uninfected mice compared with that of infected mice.
J. Counihan, B. Fried, J. Sherma, Parasitol. Res. 107 (2010) 947–953.