Aegle marmelos (Rutaceae) has gained traditional therapeutic importance owing to their coumarins (umbelliferone, psoralene, marmin, and imperatorin) and alkaloid (skimmianine). Presently, there is no appropriate thin-layer chromatography (TLC) based method available for simultaneous determination important coumarins and alkaloids of A. marmelos. A simple, sensitive, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the determination of marmin, skimmianine, umbelliferone, psoralene, and imperatorin for in the methanolic extract of root bark of A. marmelos. Analysis was performed by using TLC precoated silica gel 60 F 254 aluminium plates (20 × 10 cm) with toluene-ethyl acetate-formic acid (6:4:0.1, v/v) as mobile phase. Quantitative evaluation of the plate was performed in the absorption mode at 304 nm. The calibration curves were prepared in the concentration range of 40–500 ng spot−1 for marmin, skimmianine, and umbelliferone, for psoralene in the range of 16–200 ng spot−1 and for imperatorin in the range of 20–250 ng spot−1. The method was validated for precision, repeatability, and accuracy. The technique has been applied, for the first time, for the simultaneous estimation of marmin, skimmianine, umbelliferone, psoralene, and imperatorin. The proposed method was found to be robust, precise, and accurate; it therefore holds potential for detection, monitoring, and quantification of marmin, skimmianine, umbelliferone, psoralene, and imperatorin in A. marmelos raw root drug materials and chemical screening of germplasms.
S. Arumugam, S. Kavimani, B. Kadalmani, ABA Ahmed, M.A Akbarshac, M.V. Rao, Sci. Asia 34 (2008) 317–321.
Rao M.V., '' (2008) 34Sci. Asia: 317-321.
Rao M.V.Sci. Asia200834317321)| false
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