JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 25: Issue 4
HPTLC quantification and antimicrobial activity of ursolic acid from Diospyros melanoxylon
Authors:
Kedar Rout North Orissa University P.G. Department of Chemistry Sriramchandra Vihar, Baripada, Mayurbhanja 757003 Orissa India

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,
Bandita Dash Institute of Minerals and Materials Technology HDBR Cell Bhubaneswar 751013 Orissa India

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, and
Rajesh Singh North Orissa University P.G. Department of Chemistry Sriramchandra Vihar, Baripada, Mayurbhanja 757003 Orissa India

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Pages:
320–325
Online Publication Date:
31 Jul 2012
Publication Date:
01 Aug 2012
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.25.2012.4.7
Keywords:
HPTLC; Ursolic acid; Antimicrobial activity
Restricted access

An important bioactive molecule, ursolic acid was isolated from the leaves of Diospyros melanoxylon and characterized with help of physical and spectroscopic data viz. m. p, IR, 1H, and 13C NMR. A high-performance thin-layer chromatography method has also been developed and validated for its quantification in D. melanoxylon leaves. The high-performance thin-layer chromatography analysis was performed on high performance thin-layer chromatography plates using chloroform-methanol (9.5:0.5, v/v) as mobile phase. The compound was quantified at 540 nm after derivatzation with sulphuric acid reagent. The sensitivity of the method with respect to limit of detection and limit of quantification were found to be 20 and 40 ng per spot. The response was obtained as a linear function of peak area and concentration in the range of 50 to 450 ng per spot with correlation coefficient of r2 = 0.9998. The method showed excellent accuracy greater than 97.54% with acceptable precession and was successfully validated according to International Conference of Harmonization protocols. Antimicrobial screenings of ursolic acid revealed potent activity against two Gram-positive bacteria viz. Staphylococcus aureus and Enterococcus faecalis and three fungal starins viz. Aspergillus niger, Candida tropicalis, and Candida albicans.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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