We will present the first example of a two-dimensional scanned TLC-plate, measured by use of a diode-array scanner. A spatial resolution of 250 μm was achieved on plate. The system provides real 2D fluorescence and absorption spectra in the wavelength-range from 190 to 1000 nm with a spectral resolution of greater than 1 nm. A mixture of 12 sulphonamides was separated by using a cyanopropyl-coated silica gel plate (Merck, 1.16464) with the solvent mix of methyl tert-butyl ether-methanol-dichloromethane-cyclohexane-NH3 (25%) (48:2:2:1:1, v/v) in the first and with a mixture of water-acetonitrile-dioxane-ethanol (8:2:1:1, v/v) in the second direction. Both developments were carried out over a distance of 70 mm. A separation number (spot capacity) of 259 was calculated. We discussed a new formula for its calculation in 2D-TLC separations. The drawback of this method is that measuring a 2D-TLC plate needs more than 3 h measurement time.
B. Spangenberg, C.F. Poole, C. Weins, Quantitative Thin Layer Chromatography. A Practical Survey, Springer, Berlin, 2010.
Weins C., '', in Quantitative Thin Layer Chromatography. A Practical Survey, (2010) -.
Weins C.Quantitative Thin Layer Chromatography. A Practical Survey2010)| false
L. Mondello, A.C. Lewis, K.D. Bartle (eds), Multidimensional Chromatography, Wiley, Chichester, 2002.
Bartle K.D., '', in Multidimensional Chromatography, (2002) -.