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Miuţa Filip Babeo-Bolyai University “Raluca Ripan” Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Mihaela Vlassa Babeo-Bolyai University “Raluca Ripan” Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Florina Copaciu Babeo-Bolyai University “Raluca Ripan” Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Virginia Coman Babeo-Bolyai University “Raluca Ripan” Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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The profiles of anthocyanins and anthocyanidins present in berry fruits can be used as fingerprints for the evaluation of authenticity of raw materials, juices, fruit extracts, and food products. The aim of our work was to establish the authenticity of fruit juices from the market according to the label inscription. With this end in view, we used spectroscopic and TLC techniques for the preliminary identification of main groups of anthocyanins and to obtain a fingerprint of each berry fruit used in the preparation of juice. For the same purpose, we achieved a RP-HPLC method for the determination in detail of the composition of natural pigments, such as anthocyanins from alcoholic extracts of colored fruits and anthocyanidins from their hydrolyzed products. The RP-HPLC chromatographic fingerprints gave us the possibility to identify seventeen anthocyanins (glycosylated anthocyanidins) and five major anthocyanidins (delphinidin, cyanidin, petunidin, pelargonidin, and malvidin) from the extracts of colored fruits.The TLC separation was achieved on cellulose pre-coated plates developed with concentrated hydrochloric acid-glacial acetic acid-water as mobile phase followed by densitometric measurements in visible. The HPLC separation was carried out on ODS-2 Hypersil column by gradient elution and detection at 520 nm. The mobile phase was a mixture of acetonitrile and phosphoric acid buffer in different ratios.Our methods have been successfully applied to find the fingerprint of anthocyanins and anthocyanidins of eight berry fruits which we used as reference materials at the analysis of fruit juices from market in order to establish their authenticity and conformity with the label inscription.

  • W. Steglich, B. Fugmann, S. Lang-Fugmann (eds.), RÖMPP Encyclopedia, Natural Products, Georg Thieme Verlag, Stuttgart, 2000, pp. 40–41.

    Lang-Fugmann S. , '', in RÖMPP Encyclopedia, Natural Products , (2000 ) -.

  • J.G. Kirchner, Thin-Layer Chromatography. In: E.S. Perry (ed.), Techniques of Chemistry. 2nd edn., Vol. XIV, John Wiley & Sons, New York, 1978.

    Kirchner J.G. , '', in Techniques of Chemistry , (1978 ) -.

  • A. Castaneda-Ovando, M.L. Pacheco-Hernandez, M.E. Paez-Hernandez, J.A. Rodriguez, C.A. Galan-Vidal, Food Chem. 113 (2009) 859–871.

    Galan-Vidal C.A. , '' (2009 ) 113 Food Chem. : 859 -871 .

  • C.F. Timberlake, P. Bridle, Distribution of Anthocyanins in Food Plants. In: P. Markakis (ed.), Anthocyanins as Food Colors. Academic Press, New York, 1982.

    Bridle P. , '', in Anthocyanins as Food Colors , (1982 ) -.

  • C. García-Viguera, P. Zafrilla, F.A. Tomás-Barberán, J. Sci. Food Agric. 73 (1997) 207–213.

    Tomás-Barberán F.A. , '' (1997 ) 73 J. Sci. Food Agric. : 207 -213 .

  • C. Santos-Buelga, G. Williamson, Methods in Polyphenol Analysis. The Royal Society of Chemistry, Cambridge, 2003.

    Williamson G. , '', in Methods in Polyphenol Analysis , (2003 ) -.

  • G.W. Francis, Ř.M. Andersen, Natural Pigments. In: J. Sherma, B. Fried (eds.), Handbook of Thin Layer Chromatography. 3rd edn., Revised and Expanded. Marcel Dekker, New York, 2003, pp. 703–708.

    Andersen M. , '', in Handbook of Thin Layer Chromatography , (2003 ) -.

  • J. Bakker, P. Bridle, A. Koopman, J. Sci. Food Agric. 60 (2006) 471–476.

    Koopman A. , '' (2006 ) 60 J. Sci. Food Agric. : 471 -476 .

  • D. Burdulis, L. Ivanauskas, V. Dirsé, S. Kaslauskas, A. Ražukas, Medicina (Kaunas) 43 (2007) 971–977.

    Ražukas A. , '' (2007 ) 43 Medicina (Kaunas) : 971 -977 .

  • X. Liu, G. Xiao, W. Chen, Z. Xu, J. Wu, J. Biomed. Biotechnol. 5 (2004) 326–331.

    Wu J. , '' (2004 ) 5 J. Biomed. Biotechnol. : 326 -331 .

  • M. Jordheim, N.H. Giske, Ř.M. Andersen, Biochem. Syst. Ecol. 35 (2007) 153–159.

    Andersen M. , '' (2007 ) 35 Biochem. Syst. Ecol. : 153 -159 .

  • C. García-Viguera, P. Zafrilla, F.A. Tomás-Barberán, Food Sci. Technol. Int. 5 (1999) 487–492.

    Tomás-Barberán F.A. , '' (1999 ) 5 Food Sci. Technol. Int. : 487 -492 .

  • F. Galvano, The Chemistry of Anthocyanins. International Food Information Service, 2005; www.foodsciencecentral.com

  • R.E. Wrolstad, R.W. Durst, J. Lee, Trends Food Sci. Technol. 16 (2005) 423–428.

    Lee J. , '' (2005 ) 16 Trends Food Sci. Technol. : 423 -428 .

  • F. Betsui, N. Tanaka-Nishikawa, K. Shimomura, Plant Biotechnol. 21 (2004) 387–391.

    Shimomura K. , '' (2004 ) 21 Plant Biotechnol. : 387 -391 .

  • K. Lopes-da-Silva, S. De Pascual-Teresa, J. Rivas-Gonzalo, C. Santos-Buelga, Eur. Food Res. Technol. 214 (2002) 248–253.

    Santos-Buelga C. , '' (2002 ) 214 Eur. Food Res. Technol. : 248 -253 .

  • Y. Yoshida, H. Tamura, J. Japan. Soc. Hort. Sci. 74 (2005) 36–41.

    Tamura H. , '' (2005 ) 74 J. Japan. Soc. Hort. Sci. : 36 -41 .

  • M.C.N. Nunes, J.K. Brecht, A.M.M.B. Morais, S.A. Sargent, J. Sci. Food Agric. 86 (2006) 180–190.

    Sargent S.A. , '' (2006 ) 86 J. Sci. Food Agric. : 180 -190 .

  • K. Aaby, G. Skrede, R.E. Wrolstad, J. Agric. Food Chem. 53 (2005) 4032–4040.

    Wrolstad R.E. , '' (2005 ) 53 J. Agric. Food Chem. : 4032 -4040 .

  • R. Karbovskaya, I. Borys, Z. Hromatogr. tov. VIII(3, 4) (2008) 13–33 (in Russian).

    Borys I. , '' (2008 ) VIII Z. Hromatogr. tov. : 13 -33 .

  • P. Comandini, G. Blanda, A. Cardinali, L. Cerretani, A. Bendini, M.F. Caboni, J. Sep. Sci. 31 (2008) 3257–3264.

    Caboni M.F. , '' (2008 ) 31 J. Sep. Sci. : 3257 -3264 .

  • A. Karcher, Z. El Rassi, J. Liq. Chromatogr. Rel. Technol. 21 (1998) 1411–1432.

    Rassi Z. , '' (1998 ) 21 J. Liq. Chromatogr. Rel. Technol. : 1411 -1432 .

  • E.S. de Brito, M.C.P de Araújo, R.E. Alves, C. Carkeet, B.A. Clevidence, J.A. Novotny, J. Agric. Food Chem. 55 (2007) 9389–9394.

    Novotny J.A. , '' (2007 ) 55 J. Agric. Food Chem. : 9389 -9394 .

  • X. Wu, R.L. Prior, J. Agric. Food Chem. 53 (2005) 3101–3113.

    Prior R.L. , '' (2005 ) 53 J. Agric. Food Chem. : 3101 -3113 .

  • Q. Wua, Y. Xua, J.E. Simona, M.G. Ferruzzic, L. Hod, G.M. Pasinettid, J. Funct. Foods (2012); http://dx.doi.org/10.1016/j.jff.2012.04.010

  • N. Mateus, J.M. Machado, V. de Freitas, J. Sci. Food Agric. 82 (2002) 1689–1696.

    Freitas V. , '' (2002 ) 82 J. Sci. Food Agric. : 1689 -1696 .

  • C. Qin, Y. Li, W. Niu, Y. Ding, R. Zhang, X. Shang, Czech J. Food Sci. 28 (2010) 117–126.

    Shang X. , '' (2010 ) 28 Czech J. Food Sci. : 117 -126 .

  • J. Lee, R.W. Durst, R.E. Wrolstad, J. Food Sci. 67 (2002) 1660–1667.

    Wrolstad R.E. , '' (2002 ) 67 J. Food Sci. : 1660 -1667 .

  • D. Burdulis, V. Janulis, A. Milašius, V. Jakštas, L. Ivanauskas, J. Liq. Chromatogr. Rel. Technol. 31 (2008) 850–864.

    Ivanauskas L. , '' (2008 ) 31 J. Liq. Chromatogr. Rel. Technol. : 850 -864 .

  • I. OEcibisz, M. Mitek, Acta Sci. Pol. Technol. Aliment. 6 (2007) 75–82.

    Mitek M. , '' (2007 ) 6 Acta Sci. Pol. Technol. Aliment. : 75 -82 .

  • J. Bakker, P. Bridle, A. Koopman, J. Sci. Food Agr. 60 (2006) 471–476.

    Koopman A. , '' (2006 ) 60 J. Sci. Food Agr. : 471 -476 .

  • A. Stój, Z. Targoński, A. Malik, Acta Sci. Pol. Technol. Aliment. 5 (2006) 73–85.

    Malik A. , '' (2006 ) 5 Acta Sci. Pol. Technol. Aliment. : 73 -85 .

  • H.M. Merken, G.R. Beecher, J. Agric. Food Chem. 48 (2000) 577–599.

    Beecher G.R. , '' (2000 ) 48 J. Agric. Food Chem. : 577 -599 .

  • M. Naczk, F. Shahidi, J. Chromatogr. A 1054 (2004) 95–111.

    Shahidi F. , '' (2004 ) A1054 J. Chromatogr. : 95 -111 .

  • A. Bąkowska-Barczak, Pol. J. Food Nutr. Sci. 14/55 (2005) 107–116.

    Bąkowska-Barczak A. , '' (2005 ) 14/55 Pol. J. Food Nutr. Sci. : 107 -116 .

  • R.W. Durst, R.E. Wrolstad, Current Protocols in Food Analytical Chemistry, Separation and Characterization of Anthocyanins by HPLC, Unit F1.3, Suppl. 4, F 1.3.1-F 1.3.13. http://www.nshtvn.org/ebook/molbio/Current%20Protocols/CPFAC/faf0103.pdf

  • Ř.M. Andersen, G.W. Francis, J. Chromatogr. 318 (1985) 450–454.

    Francis G.W. , '' (1985 ) 318 J. Chromatogr. : 450 -454 .

  • M. Dossett, J. Lee, C.E. Finn, J. Funct. Foods 3 (2011) 207–214.

    Finn C.E. , '' (2011 ) 3 J. Funct. Foods : 207 -214 .

  • F. Blando, C. Gerardi, I. Nicoletti, J. Biomed. Biotechnol. 5 (2004) 253–258.

    Nicoletti I. , '' (2004 ) 5 J. Biomed. Biotechnol. : 253 -258 .

  • V. Lohachoompol, G. Srzednicki, J. Craske, J. Biomed. Biotechnol. 5 (2004) 248–252.

    Craske J. , '' (2004 ) 5 J. Biomed. Biotechnol. : 248 -252 .

  • H-J Fan-Chiang, R.E. Wrolstad, J. Food Sci. 70 (2005) C198–C202.

    Wrolstad R.E. , '' (2005 ) 70 J. Food Sci. : C198 -C202 .

  • F. Pomar, M. Novo, A. Masa, J. Chromatogr. A 1094 (2005) 34–41.

    Masa A. , '' (2005 ) 1094 J. Chromatogr. A : 34 -41 .

  • Y. Yoshida, H. Tamura, N. Koyama, ISHS Acta Horticulturae 522, XXV International Horticultural Congress (2000) ISBN 978-90-66058-63-7.

  • T. Frossen, S. Rayyan, Ř.M. Andersen, Phytochemistry 64 (2004) 1421–1428.

    Andersen M. , '' (2004 ) 64 Phytochemistry : 1421 -1428 .

  • V. Šimunič, S. Kovaè, D. GaŠo-Sokaè, W. Pfannhauser, M. Murkovic, Eur. Food Res. Technol. 220 (2005) 575–578.

    Murkovic M. , '' (2005 ) 220 Eur. Food Res. Technol. : 575 -578 .

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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