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Kalailingam Pazhanichamy Periyar Maniammai University Department of Biotechnology Vallam 613403 Tamil Nadu India

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Kannaian Bhuvaneswari Periyar Maniammai University Department of Biotechnology Vallam 613403 Tamil Nadu India

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Balasubramanian Kunthavai Periyar Maniammai University Department of Biotechnology Vallam 613403 Tamil Nadu India

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Tamilmani Eevera Tamil Nadu Agricultural University Dryland Agricultural Research Station Chettinad 630102 India

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Kaliaperumal Rajendran Periyar Maniammai University Department of Biotechnology Vallam 613403 Tamil Nadu India

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In the present study, an attempt has been made to isolate, characterize, and to quantify diosgenin from sapogenin extract of Costus igneus rhizome. Crude sapogenin extract of C. igneus rhizome was chromatographed on silica gel column, eluting with solvent mixtures of increasing polarity composed of n-hexane, chloroform, ethyl acetate (ethyl acetate up to 80%), and the fractions were collected. The structure of the isolated compound was established on the basis of elemental analysis and spectroscopic evidences (infrared [IR], proton nuclear magnetic resonance [1H NMR], carbon nuclear magnetic resonance [13C NMR], mass spectrometry [MS]). Diosgenin was quantified by high-performance thin-layer chromatography (HPTLC) (n-hexane-ethyl acetate 7:3 [v/v] as mobile phase) at RF 0.27 which gave single peak at 254 nm (after derivatization — 433 nm). The amount of diosgenin was estimated by comparing the peak area of standard to the peak area of diosgenin present in the C. igneus rhizome extract (sapogenin extract). The diosgenin present in the sapogenin extract was estimated to be 0.5%.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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