Authors:
Nada Abdelwahab Bani-Suef University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Alshaheed Shehata Ahmad Hegazy St. 62514 Beni-Suef Egypt

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Eglal Abdelaleem Bani-Suef University Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy Alshaheed Shehata Ahmad Hegazy St. 62514 Beni-Suef Egypt

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This research article describes a simple and sensitive thin-layer chromatographic (TLC)-densitometric method for simultaneous determination of guaifenesin (GUF) and pseudoephedrine HCl (PSH) in their binary mixtures and in the presence of guaifenesin impurity and related substance, guaiacol. The proposed chromatographic method has been developed using silica gel plates 60 F254 as a stationary phase with hexane-acetone-ethyl acetate-triethylamine-water (3:4:3:0.1:0.3, by volume) as a developing system followed by densitometric measurements at 208 (for pseudoephedrine HCl and guaifenesin) and 278 nm (for guaiacol). Experimental conditions have been optimized to get the desired separation and sensitivity. Calibration curves for the three studied components were constructed using polynomial equation which was superior to linear equation with respect to correlation coefficients and quantified ranges (14–25, 2–12, and 0.1–1.1 μg band−1) for pseudoephedrine HCl, guaifenesin and guaiacol, respectively. The developed method has been successfully applied for determination of pseudoephedrine HCl and guaifenesin in Triaminic® Chest Congestion syrup where no interference from syrup additives has been found. The data obtained from method validation confirm the specificity, sensitivity and accuracy of the method. Statistical comparison of the results obtained by the proposed chromatographic method and of those obtained by the reported chemometric technique showed no significant difference at 95% confidence limit.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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