Biomphalaria glabrata snails were infected with Schistosoma mansoni and maintained at different dilutions of artificial ocean water for up to 4 weeks. Glucose and maltose concentration of the digestive gland-gonad complex were analyzed by high-performance thin-layer chromatography at different stages of the infection. B. glabrata snails were divided into three experimental groups: Group A, snails with early prepatent infection (10 days post-infection); Group B, snails with late prepatent infection (22 days post-infection); and Group C, snails with patent infection (45 days post-infection). Infected snails in A were maintained at different salinities for 2 weeks and then necropsied, and their two main simple sugars, i.e., glucose and maltose, were analyzed. Groups B and C contained two subgroups: the first subgoups were analyzed after 2 weeks, and the second after 4 weeks. Controls for these experiments were maintained identically in either deionized water or artificial spring water. Maltose and glucose were extracted from the digestive gland-gonad complex in ethanol-water (70:30). 1-Butanol-glacial acetic acid-diethyl ether-deionized water (27:18:5:3) mobile phase was used to separate sugars on EMD Millipore silica gel preadsorbent plates. Sugars were detected using α-naphthol-sulfuric acid reagent and quantified with a CAMAG TLC Scanner 3 at 515 nm. The obtained data were compared using analysis of variance (ANOVA) single factor statistical analysis. Statistical differences were not found in any sugars in Group A snails. For glucose, a significant difference was found after 4 weeks in both B and C snails. For maltose, a significant difference was found after 4 weeks in B snails and after 2 weeks in C snails. Different salinity levels affect the maltose and glucose concentrations of adult B. glabrata snails infected with S. mansoni.
W.D. Williams, AMBIO 16 (1987) 180–185.
Williams W.D., '' (1987) 16AMBIO: 180-185.
Williams W.D.AMBIO198716180185)| false
B.T. Hart, P. Bailey, R. Edwards, K. Hortle, K. James, A. McMahon, C. Meredith, K. Swadling, Water Res. 24 (1990) 1103–1117.
Swadling K., '' (1990) 24Water Res.: 1103-1117.
Swadling K.Water Res.19902411031117)| false
A. Studer, R. Poulin, J. Exp. Mar. Biol. Ecol. 412 (2012) 110–116.
Poulin R., '' (2012) 412J. Exp. Mar. Biol. Ecol.: 110-116.
Poulin R.J. Exp. Mar. Biol. Ecol.2012412110116)| false