View More View Less
  • 1 Medical University of Lublin Department of Inorganic Chemistry 4a Chodźki Street 20-093 Lublin Poland
Restricted access

Two-dimensional separations were performed on polar bonded stationary phase of type cyanopropyl-silica and diol-silica by use of non-aqueous eluents (polar modifier dissolved in n-heptane) as the first direction eluents and aqueous eluents (organic modifier — MeOH dissolved in water) as the second direction eluents. The chromatographic process was performed in micro scale using 5 × 5 cm plates, small volumes of eluents, and 10 μL of plant extracts to obtain satisfying separation. Plates developed in horizontal chambers were dried and observed in UV light (254 nm and 366 nm) photographed by digital camera and derivatized by 1,1-diphenyl-2-picrylhydrazyl (DPPH) to detect antioxidants (free radical scavengers) or derivatized by Naturstoff reagent to detect phenolic compounds (characteristic luminescence of some phenolic compounds). The above experiments give the possibility to construct fingerprints for investigated Mentha species and varieties and preparations containing the herb. It can be used in quality control of the plant material and its antioxidative activity.

  • P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, M.A. Bartoszuk, E. Włodarczyk, M.J. Baran, J. Steroid Biochem. Molecular Biol. 127 (2011) 418–427.

    Baran M.J. , '' (2011 ) 127 J. Steroid Biochem. Molecular Biol. : 418 -427.

  • P.K. Zarzycki, M.B. Zarzycka, V.L. Clifton, J. Adamski, B.K. Głód, J. Chromatogr. A 1218 (2011) 5694–5704.

    Głód B.K. , '' (2011 ) 1218 J. Chromatogr. A : 5694 -5704.

  • P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, E. Włodarczyk, M.J. Baran, Anal. Chim. Acta 716 (2012) 54–60.

    Baran M.J. , '' (2012 ) 716 Anal. Chim. Acta : 54 -60.

  • P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, E. Włodarczyk, M.J. Baran, Anal. Chim. Acta 688 (2011) 168–174.

    Baran M.J. , '' (2011 ) 688 Anal. Chim. Acta : 168 -174.

  • D.B. Faber, J. Chromatogr. A 142 (1977) 421–430.

    Faber D.B. , '' (1977 ) 142 J. Chromatogr. A : 421 -430.

  • V.M. Dembitsky, J. Chromatogr. 436 (1988) 467–473.

    Dembitsky V.M. , '' (1988 ) 436 J. Chromatogr. : 467 -473.

  • M. Veit, Dtsch. Apoth. Ztg. 136 (1988) 467–473.

    Veit M. , '' (1988 ) 136 Dtsch. Apoth. Ztg. : 467 -473.

  • P.K. Zarzycki, J. Chromatogr. A 1187 (2008) 250–259.

    Zarzycki P.K. , '' (2008 ) 1187 J. Chromatogr. A : 250 -259.

  • M. Nishizawa, M. Kohno, M. Nishimura, A. Kitagawa, Y. Niwano, Chem. Pharm. Bull. (Tokyo) 53 (2005) 714–716.

    Niwano Y. , '' (2005 ) 53 Chem. Pharm. Bull. (Tokyo) : 714 -716.

  • M.A. Hawrył, M. Robak, M. Waksmundzka-Hajnos, J. Chromatogr. A 1218 (2011) 2812–2819.

    Waksmundzka-Hajnos M. , '' (2011 ) 1218 J. Chromatogr. A : 2812 -2819.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2021 0 0 0
Feb 2021 0 0 0
Mar 2021 0 0 0
Apr 2021 3 0 0
May 2021 1 0 0
Jun 2021 1 0 0
Jul 2021 0 0 0