JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 26: Issue 2
In-situ clean-up and OPLC fractionation of chamomile flower extract to search active components by bioautography
Authors:
Emil Mincsovics Corvinus University Department of Genetics and Plant Breeding, Faculty of Horticultural Sciences Villanyi Str. 29-45 H-1118 Budapest Hungary

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Péter Ott Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research Herman O. Str. 15 H-1022 Budapest Hungary

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Ágnes Alberti Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Andrea Böszörményi Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Éva Héthelyi Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Éva Szőke Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Ágnes Kéry Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Éva Lemberkovics Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary

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Ágnes Móricz Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research Herman O. Str. 15 H-1022 Budapest Hungary

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Pages:
172–179
Online Publication Date:
25 Mar 2013
Publication Date:
01 Apr 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.26.2013.2.12
Keywords:
Chamomile (Matricaria recutita L.); Antibacterial activity; Overpressured layer chromatography, OPLC; Combined on-line/off-line OPLC; GC-MS; HPLC-MS/MS; Off-line OPLC-MS
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Bioassay-guided isolation of antibacterial components of chamomile flower methanol extract was performed by overpressured layer chromatography (OPLC) with on-line detection, fractionation combined with sample clean-up in-situ in the adsorbent bed after off-line sample application. The antibacterial effect of the eluted fractions and of those compounds remaining on the adsorbent layer after separation was tested with direct bioautography (DB) against the bioluminescent Pseudomonas savastanoi pv. maculicola and Vibrio fischeri. The fractions with high biological activity were analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) and liquid chromatography and tandem mass spectrometry (LC-MS/MS). Two active uneluted compounds were characterized by off-line OPLC-MS using a thin-layer chromatography (TLC)-MS interface. Mainly, essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were identified in the active fractions.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Manuscript Submission: HERE

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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