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  • 1 Corvinus University Department of Genetics and Plant Breeding, Faculty of Horticultural Sciences Villanyi Str. 29-45 H-1118 Budapest Hungary
  • | 2 Hungarian Academy of Sciences Plant Protection Institute, Centre for Agricultural Research Herman O. Str. 15 H-1022 Budapest Hungary
  • | 3 Semmelweis University Department of Pharmacognosy, Faculty of Pharmacy Ulloi Str. 26 H-1085 Budapest Hungary
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Bioassay-guided isolation of antibacterial components of chamomile flower methanol extract was performed by overpressured layer chromatography (OPLC) with on-line detection, fractionation combined with sample clean-up in-situ in the adsorbent bed after off-line sample application. The antibacterial effect of the eluted fractions and of those compounds remaining on the adsorbent layer after separation was tested with direct bioautography (DB) against the bioluminescent Pseudomonas savastanoi pv. maculicola and Vibrio fischeri. The fractions with high biological activity were analyzed by solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) and liquid chromatography and tandem mass spectrometry (LC-MS/MS). Two active uneluted compounds were characterized by off-line OPLC-MS using a thin-layer chromatography (TLC)-MS interface. Mainly, essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were identified in the active fractions.

  • World Health Organization, Monographs on Selected Medicinal Plants, Vol. 1, Flos Chamomillae. WHO, Geneva, 1999.

    '', in Monographs on Selected Medicinal Plants, Vol. 1 , (1999 ) -.

  • J.K. Srivastava, S. Gupta, in: R.R. Watson (ed.) Complementary and Alternative Therapies in the Aging Population, Elsevier Inc., Academic Press, Amsterdam, 2009.

    Gupta S. , '', in Complementary and Alternative Therapies in the Aging Population , (2009 ) -.

  • D.L. McKay, J.B. Blumberg, Phytother. Res. 20 (2006) 519–530.

    Blumberg J.B. , '' (2006 ) 20 Phytother. Res. : 519 -530.

  • É. Szőke, E. Máday, S.A. Kiss, L. Sonnewend, É. Lemberkovics, J. Am. Coll. Nutr. 23 (2004) 763S–767S.

    Lemberkovics , '' (2004 ) 23 J. Am. Coll. Nutr. : 763S -767S.

  • É. Szőke, E. Máday, E. Tyihák, I.N. Kuzovkina, É. Lemberkovics, J. Chromatogr. B. 800 (2004) 231–238.

    Lemberkovics , '' (2004 ) 800 J. Chromatogr. B. : 231 -238.

  • M. Repcák, J. Imrich, M. Franeková, J. Plant Physiol. 158 (2001) 1085–1087.

    Franeková M. , '' (2001 ) 158 J. Plant Physiol. : 1085 -1087.

  • R. Guimarăes, L. Barros, M. Dueńas, R.C. Calhelha, A.M. Carvalho, C. Santos-Buelga, M.R.P. Queiroz, I.C.F.R. Ferreira, Food Chem. 136 (2013) 947–954.

    Ferreira I.C.F.R. , '' (2013 ) 136 Food Chem. : 947 -954.

  • N. Mulinacci, A. Romani, P. Pinelli, F.F. Vincieri, D. Prucher, Chromatographia 51 (2000) 301–307.

    Prucher D. , '' (2000 ) 51 Chromatographia : 301 -307.

  • L. Nováková, A. Vildová, J.P. Mateus, T. Gonçalves, P. Solich, Talanta 82 (2010) 1271–1280.

    Solich P. , '' (2010 ) 82 Talanta : 1271 -1280.

  • V. Svehliková, R.N. Bennett, F.A. Mellon, P.W. Needs, S. Piacente, P.A. Kroon, Y. Bao, Phytochemistry 65 (2004) 2323–2332.

    Bao Y. , '' (2004 ) 65 Phytochemistry : 2323 -2332.

  • É. Szőke, E. Máday, J. Gershenzon, J.L. Allen, É. Lemberkovics, J. Chromatogr. Sci. 42 (2004) 229–233.

    Lemberkovics , '' (2004 ) 42 J. Chromatogr. Sci. : 229 -233.

  • M. Berry, Pharm. J. 254 (1995) 191–193.

    Berry M. , '' (1995 ) 254 Pharm. J. : 191 -193.

  • M.E. Aggag, R.T. Yousef, Planta Med. 22 (1972) 140–144.

    Yousef R.T. , '' (1972 ) 22 Planta Med. : 140 -144.

  • N.C. Silva, L. Barbosa, L.N. Seito, A. Fernandes Jr., Nat Prod Res. 26 (2012) 1510–1514.

    Fernandes A. , '' (2012 ) 26 Nat Prod Res. : 1510 -1514.

  • S. Petronilho, M. Maraschin, M.A. Coimbra, S.M. Rocha, Ind. Crops Prod. 40 (2012) 1–12.

    Rocha S.M. , '' (2012 ) 40 Ind. Crops Prod. : 1 -12.

  • R. Franke, H. Schilcher (eds.) Chamomile: Industrial Profiles, II Series: Medicinal and Aromatic Plants, Vol. 42, CRC Press/Taylor & Francis Group, Boca Raton/Broken Sound Parkway, NW, 2005.

    '', in Chamomile: Industrial Profiles, II Series: Medicinal and Aromatic Plants, Vol. 42 , (2005 ) -.

  • Á.M. Móricz, S. Szarka, P. Ott, D. Kertesy, É.B. Héthelyi, É.B. Szőke, E. Tyihák, J. Planar Chromatogr. 25 (2012) 220–224.

    Tyihák E. , '' (2012 ) 25 J. Planar Chromatogr. : 220 -224.

  • Á.M. Móricz, Sz. Szarka, P.G. Ott, É.B. Héthelyi, É. Szőke, E. Tyihák, Med. Chem. 8 (2012) 85–94.

    Tyihák E. , '' (2012 ) 8 Med. Chem. : 85 -94.

  • E. Mincsovics, M. Garami, L. Kecskés, B. Tapa, Z. Végh, Gy. Kátay, E. Tyihák, J. AOAC Int. 82 (1999) 587–598.

    Tyihák E. , '' (1999 ) 82 J. AOAC Int. : 587 -598.

  • Á.M. Móricz, P.G. Ott, A. Böszörményi, É. Lemberkovics, E. Mincsovics, E. Tyihák, Chromatographia 75 (2012) 1–9.

    Tyihák E. , '' (2012 ) 75 Chromatographia : 1 -9.

  • E. Mincsovics, Gy. Kátay, P.G. Ott, Zs. Király-Véghely, Á.M. Móricz, E. Tyihák, Chromatographia 62(Suppl 13) (2005) S51–S56.

    Tyihák E. , '' (2005 ) 62 Chromatographia : S51 -S56.

  • E. Mincsovics, É. Sárdi, I. Velich, Gy. Kátay, E. Tyihák, J. Planar Chromatogr. 15 (2002) 280–285.

    Tyihák E. , '' (2002 ) 15 J. Planar Chromatogr. : 280 -285.

  • I.M. Choma, E.M. Grzelak, J. Chromatogr. A 1218 (2011) 2684–2691.

    Grzelak E.M. , '' (2011 ) 1218 J. Chromatogr. A : 2684 -2691.

  • J. Fan, C. Crooks, C. Lamb, Plant J. 53 (2008) 393–399.

    Lamb C. , '' (2008 ) 53 Plant J. : 393 -399.

  • E.O. King, M.K. Ward, D.E. Raney, J. Lab. Clin. Med. 44 (1954) 301–317.

    Raney D.E. , '' (1954 ) 44 J. Lab. Clin. Med. : 301 -317.

  • A.P. Desbois, V.J. Smith, Appl. Microbiol. Biotechnol. 85 (2010) 1629–1642.

    Smith V.J. , '' (2010 ) 85 Appl. Microbiol. Biotechnol. : 1629 -1642.

  • T.P. Tim Cushnie, A.J. Lamb, Int. J. Antimicrob. Agents 26 (2005) 343–356.

    Lamb A.J. , '' (2005 ) 26 Int. J. Antimicrob. Agents : 343 -356.

  • C. Cueva, M.V. Moreno-Arribas, P.J. Martín-Alvarez, G. Bills, M.F. Vicente, A. Basilio, C.L. Rivas, T. Requena, J.M. Rodríguez, B. Bartolomé, Res. Microbiol. 161 (2010) 372–382.

    Bartolomé B. , '' (2010 ) 161 Res. Microbiol. : 372 -382.

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