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  • 1 Laboratory for Food Chemistry National Institute of Chemistry Hajdrihova 19 SI-1000 Ljubljana Slovenia
  • | 2 Center for Validation Technologies and Analytics National Institute of Chemistry Hajdrihova 19 SI-1000 Ljubljana Slovenia
  • | 3 i-TLC d.o.o. Ulica padlih borcev 4 SI-1000 Ljubljana Slovenia
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In this article, our research group evaluates differences between standard thin-layer chromatography (TLC) and modern quantitative thin-layer chromatography (QTLC). Several most important reasons which transformed a simple powerful analytical technique, suitable for inorganic, organic, and biological samples, into reliable and regulatory acceptable technique in our laboratory are described. TLC has been accepted as good semi-qualitative technique, but a long and intensive developing period was necessary before QTLC became a respectable quantitative analytical technique. This breakthrough has been obtained with automation of main analytical task, standardization of analytical procedures, and application of operational qualification and performance qualification (OQ/PQ). Development of digital technology with capable microcomputers, image processing devices, big memory, new communication option, and new software enable in-line introduction of quality assurance (QA) principles in our laboratory. Agroup of new instruments, in some details, also a result of our research, enable simple and reliable analytical work. Results obtained with Automatic Developing Chamber 2 (ADC2) in very sensitive quantification of lutein and β-carotene demonstrated how controlled separation parameters significantly improved chromatographic parameters. In our experiment, relative standard deviation (RSD) of retardation factor (RF) for lutein and β-carotene spots were 3.70% and 1.65% in classic TLC chamber and only 2.48% and 0.94% in ADC2 chamber. Instruments may be qualified, and final results are then reported with reliable realistic error based on standardized analytical procedures. Implementation of QA system, analytical management, educated and trained staff, qualified instruments, and standard operating procedures ensure requisite reliability of our TLC analysis.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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