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  • 1 Central Institute of Medicinal and Aromatic Plants Analytical Chemistry Department Lucknow 226015 India
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Oroxylum indicum (Bignoniaceae) is an edible plant, and its young pods are eaten in Asian countries, especially in Thailand and Laos. In a recent study, we have reported its flavonoids as potent antitubercular chemicals of the plant. Here, we are reporting a high-performance thin-layer chromatographic method for simultaneous detection and quantification of these important flavonoids of O. indicum. Well resolved separation of marker compounds was achieved on silica gel 60 F254 plates using the mobile phase consisting of chloroform-methanol-water-formic acid (97:3:0.25:0.25, v/v) with acceptable limit of validation parameters such as linearity, sensitivity, recovery, etc. The developed method is reproducible, robust, and inexpensive without any derivatization process.

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