High-performance liquid chromatography (HPLC) has been the most popular choice for the determination of salicylic acid. This paper describes a method of normal phase isocratic planar chromatography on silica gel 60 F254 high-performance thin-layer chromatography (HPTLC) layer and measurement of fluorescence quenched zones by ultraviolet absorption scanning densitometry at 270 nm for separation of salicylic acid from dissolution media constituents. The established procedure was validated in terms of limit of detection (LOD), limit of quantitation (LOQ), linearity, recovery, and precision. The calibration function of the analyte was linear in the range 57–399 ng zone−1, and the correlation coefficient was 0.999. The limits of detection and quantitation were 25 and 57 ng zone−1. The relative standard deviations were less than 4.0% for repeatability and intermediate precision studies. The recovery was 96.8–103%.
I. Strodtholz, T. Schmidt, I. Strahinjic, U.S. Patent 2012/10207812 A1, 2012.
C. Buckley, S. Jackson, N. Kilcullen, D.M. Pavis, U.S. Patent 2012/0189684 A1, 2012.
S. Preneau, B. Dreno, J. Eur. Acad. Dermatol. Venereol. 26 (2012) 277–282.