View More View Less
  • 1 University of Concepción Department of Pharmacy, Faculty of Pharmacy P.O. Box 237 Concepción Chile
  • | 2 University of Concepción Department of Bromatology, Nutrition and Dietetic, Faculty of Pharmacy P.O. Box 237 Concepción Chile
Restricted access

A method using high-performance thin-layer chromatography (HPTLC) for quantitation of sertraline in human serum was developed and validated. It includes a liquid-liquid extraction with diethyl ether-chlorophorm (75:25, v/v) and carbamazepine as internal standard. Chromatographic separation was performed on precoated silica gel F254 HPTLC plates using a mixture of toluene-ethyl acetate-methanol-glacial acetic acid (4.5:1.5:1.0:0.5, v/v) as mobile phase. The method showed good relationship (r = 0.998) (1.00–70.00 ng/band, corresponding to 0.01 and 0.70 ng/μL in serum after extraction process and applying 10 μL to the chromatographic plates). This range includes serum concentration found in literature (0.02–0.50 ng/μL). The % RSD values of intra- and inter-assay were between 0.88–2.75 and 0.76–3.36, respectively. The limit of detection and the limit of quantification were 0.12 and 0.25 ng/band. The accuracy values were between 94.65% and 98.93%. RF for N-desmethylsertraline or norsertraline (major metabolite of sertraline), sertraline, and carbamazepine were 0.23, 0.45, and 0.71, respectively. Ten samples of patient volunteers who were using sertraline as a treatment for major depression were analyzed using the method developed and found sertraline concentrations between 0.05 and 0.32 ng/μL. In conclusion, the method is precise, accurate, selective, and sensitive for quantitative determination of sertraline in human serum, and it could be used for pharmacotherapy adherence evaluation.

  • J. Mc Namara, in: J. Hardman, L. Limbird (eds.), Las Bases Farmacológicas de la Terapéutica, Mc Graw-Hill, México, 2003.

    Mc Namara J. , '', in Las Bases Farmacológicas de la Terapéutica , (2003 ) -.

  • G. McEvoy, AHFS Drug Information, American Society of Health-System Pharmacists, Bethesda, 2009.

    McEvoy G. , '', in AHFS Drug Information , (2009 ) -.

  • S. Sweetman, Martindale, Guía Completa de Consulta Farmacoterapéutica, Pharma Editores S.L, Barcelona, 2003.

  • A. Akincigil, Med. Care 45 (2007) 363–369.

    Akincigil A. , '' (2007 ) 45 Med. Care : 363 -369.

  • S. Pampallona, P. Bollini, B. Tibaldi, B. Kupelnick, C. Munizza, Br. J. Psychiatry 180 (2002) 104–109.

    Munizza C. , '' (2002 ) 180 Br. J. Psychiatry : 104 -109.

  • K. Demyttenaere, Eur. Neuropsychopharmacol. 13 (2003) 69–75

    Demyttenaere K. , '' (2003 ) 13 Eur. Neuropsychopharmacol. : 69 -75.

  • C. Thompson, R. Preveler, D. Stephenson, J. McKendrick, Am. J. Psychiatry 157 (2000) 338–343.

    McKendrick J. , '' (2000 ) 157 Am. J. Psychiatry : 338 -343.

  • A. Vergouwen, A. Bakker, H. Burger, T. Verheij, F. Koerselman, Psychol. Med. 35 (2005) 25–33.

    Koerselman F. , '' (2005 ) 35 Psychol. Med. : 25 -33.

  • B. Sleath, K. Wurst, T. Lowery, Community Ment. Health J. 39 (2003) 359–368.

    Lowery T. , '' (2003 ) 39 Community Ment. Health J. : 359 -368.

  • L. van Dijk, E. Heerdink, D. Somai, S. van Dulmen, T. de Ridder, A. Griens, J. Bensing, BMC Health Ser. Res. 7 (2007) 51.

    Griens A. , '' (2007 ) 7 J. Bensing, BMC Health Ser. Res. : 51 -.

  • L. Osterberg, T. Blaschke, Drug Ther. 353 (2005) 487–497.

    Blaschke T. , '' (2005 ) 353 Drug Ther. : 487 -497.

  • M. Doesschate, C. Bockting, A. Schene, J. Affective Disord. 115 (2009) 167–170.

    Schene A. , '' (2009 ) 115 J. Affective Disord. : 167 -170.

  • C. R. Cantrell, M.T. Eaddy, M.B. Shah, T.S. Regan, M.C. Sokol, Med. Care 44 (2006) 300–303.

    Sokol M.C. , '' (2006 ) 44 Med. Care : 300 -303.

  • J. Aikens, D. Nease, D. Nau, M. Klinkman, T. Schwenk, Ann. Fam. Med. 3 (2005) 23–30.

    Schwenk T. , '' (2005 ) 3 Ann. Fam. Med. : 23 -30.

  • V. Jukic, Coll. Antropol. 27 (2003) 119–124.

    Jukic V. , '' (2003 ) 27 Coll. Antropol. : 119 -124.

  • M. Sajatovic, C.H. Peijun, D. Philipp, S. Edwin, Dis. Manag. Health Out. 15 (2007) 181–192.

    Edwin S. , '' (2007 ) 15 Dis. Manag. Health Out. : 181 -192.

  • L. He, F. Feng, J. Wu, J. Chromatogr. Sci. 43 (2005) 532–535.

    Wu J. , '' (2005 ) 43 J. Chromatogr. Sci. : 532 -535.

  • D. Jain, M. Sanyal, G. Subbaiah, U.C. Pande, P. Shrivastav, J. Chromatogr. B 829 (2006) 69–74.

    Shrivastav P. , '' (2006 ) 829 J. Chromatogr. B : 69 -74.

  • B. J. Goncalves, R.H. Costa Queiroz, M.E. Costa Queiroz, J. Anal. Toxicol. 31 (2007) 313–320.

    Queiroz M.E. C. , '' (2007 ) 31 J. Anal. Toxicol. : 313 -320.

  • X. Chen, X. Duan, X. Dai, D. Zhong, Rapid Commun. Mass Spectrom. 20 (2006) 4283–4289.

    Zhong D. , '' (2006 ) 20 Rapid Commun. Mass Spectrom. : 4283 -4289.

  • B. N. Patel, N. Sharma, M. Sanyal, P.S. Shrivastav, J. Chromatogr. B 877 (2009) 221–229.

    Shrivastav P.S. , '' (2009 ) 877 J. Chromatogr. B : 221 -229.

  • N. Khalil, A. Mahmoud, I. Darwish, A. Al-Majed, Chromatographia 71 (2010) 825–831.

    Al-Majed A. , '' (2010 ) 71 Chromatographia : 825 -831.

  • A. Farshchi, G. Golbarg, B. Gholamreza, Iranian J. Pharm. Sci. 5 (2009) 171–178.

    Gholamreza B. , '' (2009 ) 5 Iranian J. Pharm. Sci. : 171 -178.

  • N. Ben Omar, T. Jahner, A. Doerfelt, D. Melchner, A. Köstlbacher, E. Haen, Pharmacopsychiatry 21 (2011) A8.

    Haen E. , '' (2011 ) 21 Pharmacopsychiatry : A8 -.

  • M. Zhang, F. Gao, X. Cui, Y. Zhang, Y. Sun, J. Gu, J. Chromatogr. Sci. 49 (2011) 89–93.

    Gu J. , '' (2011 ) 49 J. Chromatogr. Sci. : 89 -93.

  • I. Darwish, H. Abdine, S. Amer, L. Al-Rayes, Int. J. Anal. Chem. 2009 (2009) 14–21.

    Al-Rayes L. , '' (2009 ) 2009 Int. J. Anal. Chem. : 14 -21.

  • M. Walash, F. Belat, N. E-Enary, H. Elmansi, Chem. Cent. J. 5 (2011) 61.

    Elmansi H. , '' (2011 ) 5 Chem. Cent. J. : 61 -.

  • I. Darwish, S. Amer, H. Abdine, L. Al-Rayes, Anal. Chem. Insights 3 (2008) 145–155.

    Al-Rayes L. , '' (2008 ) 3 Anal. Chem. Insights : 145 -155.

  • N. El-Enany, A. Abdelal, F. Belal, Chem. Cent. J. 5 (2011) 56.

    Belal F. , '' (2011 ) 5 Chem. Cent. J. : 56 -.

  • A. Musenga, E. Kenndler, L. Mercolini, M. Amore, S. Fanali, M.A. Raggi, Electrophoresis 28 (2007) 1823–1831.

    Raggi M.A. , '' (2007 ) 28 Electrophoresis : 1823 -1831.

  • K. Mee Kim, B. Hwa Jung, M. Ho Choi, J. Soo Woo, K.J. Chung, J. Chromatogr. B 769 (2002) 333–339.

    Chung K.J. , '' (2002 ) 769 J. Chromatogr. B : 333 -339.

  • I. Wilson, Ther. Drug Monit. 18 (1996) 484–492.

    Wilson I. , '' (1996 ) 18 Ther. Drug Monit. : 484 -492.

  • S. Mennickent, A. Sobarzo, M. Vega, C.G. Godoy, M. de Diego, J. Sep. Sci. 30 (2007) 2167–2172.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 2167 -2172.

  • S. Mennickent, R. Fierro, M. Vega, M. de Diego, J. Sep. Sci. 32 (2009) 1454–1458.

    Diego M. , '' (2009 ) 32 J. Sep. Sci. : 1454 -1458.

  • S. Mennickent, A. Sobarzo, M. Vega, M. de Diego, G. Godoy, P. Rioseco, L. Saavedra, Int. J. Psychiatry Clin. Pract. 14 (2010) 41–46.

    Saavedra L. , '' (2010 ) 14 Int. J. Psychiatry Clin. Pract. : 41 -46.

  • S. Mennickent, M. Vega, C.G. Godoy, M.D. León, Rev. Méd. Chile 135 (2007) 335–340.

    León M.D. , '' (2007 ) 135 Rev. Méd. Chile : 335 -340.

  • S. Mennickent, R. Fierro, M. Vega, M. de Diego, C.G. Godoy, J. Sep. Sci. 33 (2010) 2206–2210.

    Godoy C.G. , '' (2010 ) 33 J. Sep. Sci. : 2206 -2210.

  • S. Mennickent, R. Fierro, M. Vega, M. de Diego, C. Godoy, J. Planar Chromatogr. 24 (2011) 222–226.

    Godoy C. , '' (2011 ) 24 J. Planar Chromatogr. : 222 -226.

  • S. Mennickent, M. Vega, M.A. Vega, M. de Diego, R. Fierro, J. Planar Chromatogr. 25 (2012) 54–59.

    Fierro R. , '' (2012 ) 25 J. Planar Chromatogr. : 54 -59.

  • S. Mennickent, J. Contreras, B. Schulz, M. de Diego, M. Vega, Quím. Nova 35 (2012) 411–415.

    Vega M. , '' (2012 ) 35 Quím. Nova : 411 -415.

  • S. Mennickent, L. Pino, M. Vega, C.G. Godoy, M. de Diego, J. Sep. Sci. 30 (2007) 772–777.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 772 -777.

  • S. Mennickent, L. Pino, M. Vega, M. de Diego, J. Sep. Sci. 31 (2007) 201–206.

    Diego M. d. , '' (2007 ) 31 J. Sep. Sci. : 201 -206.

  • S. Mennickent, M. Nail, M. Vega, M. de Diego, J. Sep. Sci. 30 (2007) 1893–1898.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 1893 -1898.

  • S. Mennickent, J. Contreras, C. Reyes, M. Vega, M. de Diego, J. Planar Chromatogr. 23 (2010) 1–13.

    Diego M. , '' (2010 ) 23 J. Planar Chromatogr. : 1 -13.

  • G. Shabir, J. Chromatogr. A 987 (2003) 57–66.

    Shabir G. , '' (2003 ) 987 J. Chromatogr. A : 57 -66.

  • O. Quattrochi, S. Abelaira, R. Laba, Introducción a la HPLC: Aplicación y práctica. Artes Gráficas Farro, Buenos Aires, 1992.

  • W. Daniel, Bioestadística. Base para el Análisis de las Ciencias de la Salud. Uteha Noriega Editores, México, 1996.

  • The United States Pharmacopeia, U.S. Pharmacopeial Convection Inc., Rockville, 2005.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2021 0 0 0
Feb 2021 8 0 0
Mar 2021 3 0 0
Apr 2021 0 0 0
May 2021 0 0 0
Jun 2021 0 0 0
Jul 2021 0 0 0