The γ-amino butyric acid (GABA) is the main inhibitory neurotransmitter system in the brain. Either imbalance in the extreme can result in several mental diseases like schizophrenia, cerebral stroke, temporal lobe epilepsy (TLE), Parkinson’s disease (PD), Huntington’s disease (HD), and anxiety disorders even death. Measurement of GABA in tissue will help to elucidate the metabolic role and diagnostic value. Various analytical techniques are employed to estimate GABA in biological samples but the experimental procedure and tedious techniques are required. The present study demonstrates a simple, feasible, and cost-effective high-performance thinlayer chromatography (HPTLC) method for estimation of GABA in mice brain tissue. The method was validated in terms of specificity, linearity, and detection limit. The precision was done for inter-day, intra-day, instrumental, and reproducibility. Accuracy was checked by recovery study. The results indicate that this method is fast, sensitive, and suitable for quantitative assessment.
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