JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 26: Issue 6
Two-dimensional thin-layer chromatographic fingerprint of Helleborus thibetanus Franch. Using a polyamide plate with nonaqueous and reversed micellar mobile phases
Authors:
Ningwei Lu Lanzhou University School of Pharmacy Lanzhou 730000 Gansu, P. R. China

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Wenxiang Zhang Tianshui Municipal First People’s Hospital Department of Pharmacy Tianshui 741000 Gansu, P. R. China

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Qiong An Lanzhou University School of Pharmacy Lanzhou 730000 Gansu, P. R. China

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Ning Li Lanzhou University School of Pharmacy Lanzhou 730000 Gansu, P. R. China

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Yuming Dong Lanzhou University School of Pharmacy Lanzhou 730000 Gansu, P. R. China

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Pages:
463–469
Online Publication Date:
14 Jan 2014
Publication Date:
01 Dec 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.26.2013.6.1
Keywords:
Two-dimensional thin-layer chromatography; Fingerprint; Reversed micelle; Canonical correlation analysis
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A two-dimensional thin-layer chromatographic fingerprint has been developed on a polyamide plate for the quality control of Helleborus thibetanus Franch. The investigated sample was separated by chloroform-ethyl acetate-methanol (3.0:8.0:4.4, v/v) in the first dimension and isooctane-n-propyl alcohol-water (10:2.5:1.0, v/v) adding 0.28 mol L−1 sodium dodecyl sulfate, a reversed micelle, in the second dimension. The plate was dried in the air at room temperature and examined in ultraviolet (UV) light at λ = 365 nm after development. The two developments were carried out over a distance of 70 mm. The two-dimensional thin-layer chromatographic method was validated in terms of repeatability, stability, and robustness. For fingerprint analysis, nine spots were identified as common spots. Statistical method (canonical correlation analysis) was first used to calculate the degree of similarity between the two-dimensional chromatograms. The proposed method was novel and accurate for the identification and quality evaluation of H. thibetanus Franch.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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