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  • 1 University of Mysore Department of Studies in Microbiology Manasagangotri, Mysore 570 006 Karnataka India
  • | 2 Unit of Central Coffee Research Institute, Coffee Board Plant Biotechnology Division Manasagangotri, Mysore 570 006 Karnataka India
  • | 3 Bogor Agricultural University Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences Jl. Agathis 1 16680 Darmaga-Bogor Indonesia
  • | 4 University of Mysore Department of Studies in Chemistry Manasagangotri, Mysore 570 006 Karnataka India
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Rapid identification of known compounds, i.e., dereplication, has become a strategically important area for the natural-product chemists involved in bioprospecting of microbes for novel bioactive metabolites. Among microbial biodiversity, endophytic fungi represent an abundant and dependable source of structurally diverse bioactive metabolites. During the course of screening for antimicrobial secondary metabolites from endophytic fungi, an antimicrobial metabolite was identified from the ethyl acetate extract obtained from the culture broth of Xylaria sp., an endophytic fungus from Ficus pumila Linn. (Moraceae) that exhibited a broad spectrum of antimicrobial activity against human and phytopathogenic bacteria and fungi. Chemical investigation of the ethyl acetate fraction using thin-layer chromatography (TLC) bioautography and LC-hyphenated techniques led to the identification of a known benzoic acid derivative. Here, we describe the application of analytical strategies (TLC-bioautography) and hyphenated spectroscopic techniques (liquid chromatography-photodiode array detector-mass spectrometry [LC-PDA-MS]) for the dereplication of antimicrobial metabolites.

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