A simple and sensitive high-performance thin-layer chromatographic (HPTLC)-densitometric method was developed and validated for quantification of β-amyrin in the crude extracts of two species of Maytenus (Maytenus obscura and Maytenus parviflora) grown in Saudi Arabia. HPTLC-densitometry was performed on glass-backed silica gel 60 F254 TLC plates with the binary mobile phase hexane-ethyl acetate (3:1, v/v). The developed plate was derivatized with p-anisaldehyde, then scanned and quantified densitometrically at 550 nm. The system was found to give a compact spot for β-amyrin at RF value of 0.38 ± 0.01. The method was found to be satisfactory in terms of sensitivity, accuracy, precision, and recovery. The content of β-amyrin was estimated as 0.42% ± 0.01% and 0.88% ± 0.01% w/w in M. obscura and M. parviflora, respectively. The developed HPTLC technique can be very useful for the quantification of β-amyrin present in various medicinal plants.
M.S. Ahmed, H.H.S. Fong, D.D. Soejarto, R.H. Dobberstein, D.P. Waller, J. Chromatogr. 213 (1981) 340–344.
Waller DP, '' (1981) 213J. Chromatogr.: 340-344.
Waller DPJ. Chromatogr.1981213340344)| false
S.M. Kupchan, Y. Kamoda, A.R. Branfman, A.T. Sneden, W.A. Court, G.J. Thomas, H.P. Hintz, R.M. Smithl, A. Karim, G.A. Howle, A.K. Verma, Y. Nagao, J.R.G. Dailey, V.A. Zimmerly, J.W.C. Sumner, J. Org. Chem. 42 (1977) 2349–2357.
Sumner JWC, '' (1977) 42J. Org. Chem.: 2349-2357.
Sumner JWCJ. Org. Chem.19774223492357)| false
O. Munoz, C. Galeffi, E. Federacy, J.A. Garbarino, M. Piovano, M. Nicoletti, Phytochemistry 40 (1995) 853–855.
Nicoletti M, '' (1995) 40Phytochemistry: 853-855.
Nicoletti MPhytochemistry199540853855)| false
N. Zhu, N. Sharpin, J. Zhang, Phytochemistry 47 (1998) 265–268.
Zhang J, '' (1998) 47Phytochemistry: 265-268.
Zhang JPhytochemistry199847265268)| false
C.L. Queiroga, G.F. Silva, P.C. Dias, A. Possenti, J.E. de Carvelho, J. Ethnopharmacol. 72 (2000) 465–468.
Carvelho JE, '' (2000) 72J. Ethnopharmacol.: 465-468.
Carvelho JEJ. Ethnopharmacol.200072465468)| false
N. Kaul, H. Agrawal, B. Patil, A. Kakad, S.R. Dhaneshwar, IL Farmaco 60 (2005) 351–360.
Dhaneshwar SR, '' (2005) 60IL Farmaco: 351-360.
Dhaneshwar SRIL Farmaco200560351360)| false
S.I. Alqasoumi, P. Alam, A.J. Alrehaily, F. Shakeel, M.S. Abdel-Kader, J. Planar Chromatogr. 24 (2011) 48–52.
International Conference on Harmonisation (ICH) of Technical Requirements for Registration of Pharmaceuticals for Human Use: Harmonised Triplicate Guideline on Validation of Analytical Procedures: Methodology, Recommended for Adoption at Step 4 of the ICH Process by the ICH Steering Committee, IFPMA, 1996, Geneva.