JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 27: Issue 1
Ethyl Acetate-Propionic Acid as Green Mobile Phase System for Selective Densitometric Identification of Maltose with Preliminary Separation on Silica High-Performance Thin-Layer Chromatographic Plates
Authors:
Ali Mohammad Aligarh Muslim University Department of Applied Chemistry, Faculty of Engineering and Technology Aligarh India

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,
Asma Siddiq Aligarh Muslim University Department of Applied Chemistry, Faculty of Engineering and Technology Aligarh India

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,
Abdul Moheman Jamia Hamdard Department of Chemistry New Delhi India

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, and
Gaber El-Desoky King Saud University Saudi Arabia

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Pages:
19–22
Online Publication Date:
06 Feb 2014
Publication Date:
01 Feb 2014
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.27.2014.1.3
Restricted access

A mobile phase system comprising of ethyl acetate and propionic acid in 1:1 (v/v) ratio was identified as the most suitable green mobile phase for selective separation of maltose from fructose, dextrose, galactose, or mannose on precoated silica gel 60 HPTLC plates. The effect of presence of inorganic cations as impurities in the sample was examined for the separation of sugars. The chromatographic parameters like ΔRF, separation factor (α), and resolution (Rs) of separated components of the mixture of maltose-fructose, maltose-dextrose, maltose-galactose, and maltose-mannose were calculated. The limits of detection for fructose, dextrose, and mannose were 7.5 ± 0.41 μg spot−1 and for maltose and galactose were 1.5 ± 0.09 μg spot-1. The proposed method is rapid, sensitive, and free from the use of toxic organic solvents and is therefore environmentally safe. Maltose has been selectively identified in the presence of fructose, dextrose, galactose, and mannose using scanning densitometry.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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