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  • 1 Aligarh Muslim University Department of Applied Chemistry, Faculty of Engineering and Technology Aligarh India
  • 2 Jamia Hamdard Department of Chemistry New Delhi India
  • 3 King Saud University Saudi Arabia
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A mobile phase system comprising of ethyl acetate and propionic acid in 1:1 (v/v) ratio was identified as the most suitable green mobile phase for selective separation of maltose from fructose, dextrose, galactose, or mannose on precoated silica gel 60 HPTLC plates. The effect of presence of inorganic cations as impurities in the sample was examined for the separation of sugars. The chromatographic parameters like ΔR F, separation factor (α), and resolution (Rs) of separated components of the mixture of maltose-fructose, maltose-dextrose, maltose-galactose, and maltose-mannose were calculated. The limits of detection for fructose, dextrose, and mannose were 7.5 ± 0.41 μg spot−1 and for maltose and galactose were 1.5 ± 0.09 μg spot-1. The proposed method is rapid, sensitive, and free from the use of toxic organic solvents and is therefore environmentally safe. Maltose has been selectively identified in the presence of fructose, dextrose, galactose, and mannose using scanning densitometry.

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