Authors:
Sunita Shailajan Ramnarain Ruia College, Matunga (East) Herbal Research Lab Mumbai 400 019 India

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Harshada Hande Ramnarain Ruia College, Matunga (East) Herbal Research Lab Mumbai 400 019 India

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Harshvardhan Joshi Ramnarain Ruia College, Matunga (East) Herbal Research Lab Mumbai 400 019 India

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Bhavesh Tiwari Ramnarain Ruia College, Matunga (East) Herbal Research Lab Mumbai 400 019 India

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Sasikumar Menon Institute for Advanced Training and Research in Interdisciplinary Sciences 194, Scheme No. 6, Road No. 15, Sion Koliwada, Sion (E) Mumbai 400 022 India

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Marmelosin is one of the major phytochemical constituents of Aegle marmelos (L.) Corr. (Rutaceae). Till date, there are no methods reported for the estimation of marmelosin in plasma matrix using high-performance thin-layer chromatography (HPTLC) which is a versatile analytical technique promoting shorter analysis time and less consumption of solvents with efficient data acquisition and processing. This study is an attempt to develop a rapid and sensitive HPTLC method for the estimation of marmelosin from rat plasma and its application to evaluate pharmacokinetics of A. marmelos fruit pulp extract and a traditional Unani medicine Sharabat-e-Belgiri. Plasma processing involved liquid-liquid extraction with diethyl ether followed by HPTLC. Separation and determination of marmelosin were performed on silica gel 60 F254 TLC plate using toluene-ethyl acetate-glacial acetic acid as mobile phase. United States Food and Drug Administration (USFDA) guidelines for bioanalytical validation were followed to validate the HPTLC method. RF of marmelosin was found to be 0.57. The calibration curve was linear over the range of 1.0–150.0 μg mL−1 (r2 = 0.995) in rat plasma. Limit of detection and limit of quantitation were found to be 0.5 μg mL−1 and 1.0 μg mL−1, respectively. The extraction recovery from plasma was in the range of 90.07–98.22%. The intra-day and inter-day precisions were found to be 90.89–104.12%, and 92.82–104.86%, respectively. The absorption of marmelosin from extract was found significantly higher than the formulation. The results indicated that HPTLC is a suitable technique for the estimation and pharmacokinetic evaluation of marmelosin from plant extract and Unani formulation in rat plasma.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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