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Savita Chewchinda Mahidol University Department of Pharmacognosy, Faculty of Pharmacy Bangkok 10400 Thailand

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Nongluck Ruangwises Mahidol University Department of Pharmaceutical Chemistry, Faculty of Pharmacy Bangkok 10400 Thailand

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Wandee Gritsanapan Mahidol University Department of Pharmacognosy, Faculty of Pharmacy Bangkok 10400 Thailand

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A simple, rapid, and cost-effective thin-layer chromatography (TLC) image analysis was developed and validated to quantify rhein content in Cassia fistula pod pulp extract. ImageJ software was used in the proposed method, and its data were compared to TLC-densitometric data. The TLC image analysis method promoted acceptable validation parameters, showing linear relationship with r2 = 0.9988 in the concentration range of 400–1200 ng spot−1. The limit of detection (LOD) and limit of quantification (LOQ) were 84.60 and 256.36 ng spot−1, respectively. The paired t-test showed no statistically significant difference (P > 0.05) between the mean content of rhein performed by these two methods. TLC image analysis is reliable and can be used as an alternative analytical method for rhein content in C. fistula pod pulp extract and its finished products.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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