Authors:
Kedar Rout North Orissa University P.G. Department of Chemistry Sriramchandra Vihar, Baripada, Mayurbhanja 757 003 Orissa India

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Swasti Swain Utkal University, Bhubaneswar Plant Cell and Tissue Culture Facility, Post-Graduate Department of Botany 751 004 Orissa India

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Pradeep Chand Utkal University, Bhubaneswar Plant Cell and Tissue Culture Facility, Post-Graduate Department of Botany 751 004 Orissa India

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A simple normal-phase high-performance thin-layer chromatography (HPTLC) method has been developed for the quantification of β-sitosterol in hairy root cultures of Clitoria ternatea vis-à-vis natural plant parts. Chromatographic separation was achieved on normal-phase TLC plates by using optimized mobile phase of n-hexane. acetone (8:2, v/v). Densitometric scanning was performed after postchromatographic derivatization of the plate at 414 nm. The limits of detection and quantification were found to be 40 ng and 100 ng spot−1, respectively. A linear response of calibration line was observed over the range of 100 to 500 ng spot−1 with a correlation coefficient of r2 = 0.999. Recovery values from 97.22 to 98.51% with small coefficient of variation showed excellent accuracy of the method. The method was validated according to the International Conference on Harmonization (ICH) protocol. The method enables excellent separation and accurate quantification of β-sitosterol in different plant parts of the two floral varieties of C. ternatea along with their cultured roots derived through genetic transformation. β-Sitosterol content was comparable between the blue-flowered and white-flowered plants; roots had a higher content than that in stem and leaf regardless of the two floral varieties. Transformed root cultures had a lower content of β-sitosterol compared to natural roots.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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