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Bugusu Nyamweru Muhimbili University of Health and Allied Sciences Medicinal Chemistry School of Pharmacy PO Box 65013 Dar es Salaam Tanzania

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Joseph Sempombe Muhimbili University of Health and Allied Sciences Medicinal Chemistry School of Pharmacy PO Box 65013 Dar es Salaam Tanzania

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Eliangiringa Kaale

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Thomas Layloff Supply Chain Management System Arlington VA USA

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A thin-layer chromatography (TLC) method for the analysis of clotrimazole was developed and validated according to the International Conference on Harmonization (ICH) and the United States Pharmacopeia (USP) guidelines. The developed method will be used for a qualitative and quantitative analysis of clotrimazole in vaginal tablets. The method was developed using mobile phase containing toluene-acetone (15:10 v/v) on precoated TLC silica gel 60 F254 glass plates at a detection wavelength of 215 nm using reflectance absorbance and saturation time of 20 min. Densitometric analysis showed clotrimazole being retained at RF of 0.27. The method had acceptable level of specificity where no interference was observed between clotrimazole peaks to that of blank. The calibration curve of clotrimazole was estimated using both linear and polynomial regression function in the range of 1000–2400 ng spot−1 with regression coefficient, r2, of 0.992 for linear and 0.999 for polynomial regression. The accuracy at nominal concentration of clotrimazole was found to be 99.14%, and % RSD was 1.13% for repeatability and 1.30% for intermediate precision that involved two analysts. The developed analytical method is useful for the determination of clotrimazole in the finished drug formulation. Also it helps to identify substandard and fake medicines labeled as clotrimazole during postmarketing surveillance of the drug and especially in resource-constrained countries.

  • I. Haller, Am J. Obstet Gynecol. 152 (1985) 939–944.

    Haller I , '' (1985 ) 152 Am J. Obstet Gynecol. : 939 -944 .

  • B.M. Dhananjay, B.B. Shashikant, R.T. Madhukar, J. Planar Chromatogr. 21 (2008) 277–282.

    Madhukar RT , '' (2008 ) 21 J. Planar Chromatogr. : 277 -282 .

  • U. Roychowdhury, S.K. Das, J. AOAC Int. 79 (1996) 656–659.

    Das SK , '' (1996 ) 79 J. AOAC Int. : 656 -659 .

  • E.M. Abdel-Moety, F.I. Khattab, K.M. Kelani, A.M. AbouAl-Alamein, Farmaco 57 (2002) 931–938.

    AbouAl-Alamein AM , '' (2002 ) 57 Farmaco : 931 -938 .

  • P. Parul, M. Ankita, Indian J. Pharm. Sci. 71 (2009) 451–454.

    Ankita M , '' (2009 ) 71 Indian J. Pharm. Sci. : 451 -454 .

  • R.J. Ekiert, J. Krzek, Acta Pol. Pharm. 66 (2009) 19–24.

    Krzek J , '' (2009 ) 66 Acta Pol. Pharm. : 19 -24 .

  • F. Khalil, M. Ramin, J. Pharm. Biomed. Anal. 30 (2002) 1023–1033.

    Ramin M , '' (2002 ) 30 J. Pharm. Biomed. Anal. : 1023 -1033 .

  • F.C. Pereira, M.V.B. Zanoni, C.C.I Guaratini, A.G. Fogg, J. Pharm. Biomed. Anal. 27 (2002) 201–208.

    Fogg AG , '' (2002 ) 27 J. Pharm. Biomed. Anal. : 201 -208 .

  • E. Kaale, P. Risha, E. Reich, P.T. Layloff, J. AOAC Int. 93 (2010) 1836–1843.

    Layloff PT , '' (2010 ) 93 J. AOAC Int. : 1836 -1843 .

  • E. Kaale, P. Risha, P.T. Layloff, International Symposium on TLC, 6–8, July 2011, Basel, Switzerland.

  • T.A. Kenyon, A.S. Kenyon, T.B. Sibiya, WHO 72 (1994) 615–620.

    Sibiya TB , '' (1994 ) 72 WHO : 615 -620 .

  • International Programme on Chemical Safety, WHO Geneva, 1990 ( http://www.bvsde.ops-oms.org ; accessed on Dec 2012)

  • USP 29 (2006) 570.

  • www.ich.org . Accessed on September 2013

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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