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Dimal Shah Indukaka Ipcowala College of Pharmacy Beyond GIDC, P.B. No. 53, Vitthal Udyognagar 388 121 Gujarat India

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Jainika Rana Indukaka Ipcowala College of Pharmacy Beyond GIDC, P.B. No. 53, Vitthal Udyognagar 388 121 Gujarat India

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Sunil Baldania Indukaka Ipcowala College of Pharmacy Beyond GIDC, P.B. No. 53, Vitthal Udyognagar 388 121 Gujarat India

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Usmangani Chhalotiya Indukaka Ipcowala College of Pharmacy Beyond GIDC, P.B. No. 53, Vitthal Udyognagar 388 121 Gujarat India

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Kashyap Bhatt Indukaka Ipcowala College of Pharmacy Beyond GIDC, P.B. No. 53, Vitthal Udyognagar 388 121 Gujarat India

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A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of paracetamol, dicyclomine hydrochloride, and mefenamic acid in combined dosage form. The method employed high-performance thin-layer chromatography (HPTLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was toluene-ethyl acetate-methanol (6:3:1, v/v). The RF values were observed to be 0.27 ± 0.02, 0.41 ± 0.03, and 0.61 ± 0.02 for paracetamol, dicyclomine hydrochloride, and mefenamic acid, respectively. The separated spots were densitometrically analyzed in absorbance mode at 290 nm. The method was linear in the range of 300-1800 ng band−1 for paracetamol, 400–2400 ng band−1 for dicyclomine hydrochloride, and of 150–1500 ng band−1 for mefenamic acid. The method was validated with respect to accuracy, precision, specificity, sensitivity, and robustness. The limits of detection for paracetamol, dicyclomine hydrochloride, and mefenamic acid were found to be 35, 109, and 42 ng band−1, respectively. The limits of quantification for paracetamol, dicyclomine and mefenamic acid were found to be 116, 360, and 140 ng band−1, respectively. The method was successfully applied to the estimation of all three drugs in combined tablet dosage form.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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