A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of paracetamol, dicyclomine hydrochloride, and mefenamic acid in combined dosage form. The method employed high-performance thin-layer chromatography (HPTLC) aluminum plates precoated with silica gel 60 F254 as the stationary phase, while the solvent system was toluene-ethyl acetate-methanol (6:3:1, v/v). The RF values were observed to be 0.27 ± 0.02, 0.41 ± 0.03, and 0.61 ± 0.02 for paracetamol, dicyclomine hydrochloride, and mefenamic acid, respectively. The separated spots were densitometrically analyzed in absorbance mode at 290 nm. The method was linear in the range of 300-1800 ng band−1 for paracetamol, 400–2400 ng band−1 for dicyclomine hydrochloride, and of 150–1500 ng band−1 for mefenamic acid. The method was validated with respect to accuracy, precision, specificity, sensitivity, and robustness. The limits of detection for paracetamol, dicyclomine hydrochloride, and mefenamic acid were found to be 35, 109, and 42 ng band−1, respectively. The limits of quantification for paracetamol, dicyclomine and mefenamic acid were found to be 116, 360, and 140 ng band−1, respectively. The method was successfully applied to the estimation of all three drugs in combined tablet dosage form.