Simultaneous quantification of the anti-inflammatory phytoconstituents betulinic acid, 24β-ethylcholesta-5,22E,25-triene-3β-ol, and lupeol in Clerodendrum phlomidis

Akhilesh Yadav; Madan Gupta

doi:10.1556/jpc.27.2014.3.5

JPC - Journal of Planar Chromatography - Modern TLC

Volume/Issue:: Volume 27: Issue 3

Simultaneous quantification of the anti-inflammatory phytoconstituents betulinic acid, 24β-ethylcholesta-5,22E,25-triene-3β-ol, and lupeol in Clerodendrum phlomidis

Authors:

Akhilesh Yadav

Akhilesh Yadav Central Institute of Medicinal and Aromatic Plants Analytical Chemistry Department, CSIR Lucknow 226015 India

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Madan Gupta

Madan Gupta Central Institute of Medicinal and Aromatic Plants Analytical Chemistry Department, CSIR Lucknow 226015 India

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Pages:: 174–180

Online Publication Date:: 27 May 2014

Publication Date:: 01 Jun 2014

Article Category:: Research Article

DOI:: https://doi.org/10.1556/jpc.27.2014.3.5

Keywords:: Clerodendrum phlomidis ; Lupeol; 24β-Ethylcholesta-5,22E,25-triene-3β-ol; Betulinic acid; HPTLC

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A new validated high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous determination of anti-inflammatory compounds betulinic acid (BA, 1), 24β-ethylcholesta-5,22E,25-triene-3β-ol (ECTO, 2), and lupeol (LU, 3) in the roots of Clerodendrum phlomidis. Extraction efficiency of marker compounds was studied using normal (cold and hot), ultrasonic, as well as microwave-assisted extraction techniques with various solvents. Well-resolved separation of marker compounds was achieved on silica gel 60F₂₅₄ plates using the mobile phase consisting of chloroform-methanol (98:2, ½/½). Marker compounds were scanned using the densitometric reflection-absorption mode after post-chromatographic derivatization with vanillin-sulfuric acid reagent. Validation of method was performed as per the International Conference on Harmonization (ICH) guidelines. Report on the occurrence of betulinic acid for the first time in C. phlomidis is of chemotaxonomic importance. In addition, anti-inflammatory potential of the rare sterol ECTO (2) on lipopolysaccharide (LPS)-stimulated production of pro-inflammatory cytokines (tumor necrosis factor-α [TNF-α] and interleukin-6 [IL-6]) was also evaluated as it was not reported earlier.

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JPC - Journal of Planar Chromatography - Modern TLC

Print ISSN:: 0933-4173

Online ISSN:: 1789-0993

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Journal of Planar Chromatography - Modern TLC
Language	English
Size	A4
Year of Foundation	1988
Volumes per Year	1
Issues per Year	6
Founder	Akadémiai Kiadó
Founder's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher	Akadémiai Kiadó Springer Nature Switzerland AG
Publisher's Address	H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible Publisher	Chief Executive Officer, Akadémiai Kiadó
ISSN	0933-4173 (Print)
ISSN	1789-0993 (Online)