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  • 1 Medical University Department of Analytical Chemistry Chodźki 4a 20-093 Lublin Poland
  • | 2 Medical University Department of Organic Chemistry Chodźki 4a 20-093 Lublin Poland
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The purpose of this paper was to assess the suitability of thin-layer chromatography (TLC) as a pilot method for high-performance liquid chromatography (HPLC). The new 1,2,4-triazole and thiosemicarbazide derivatives were analyzed by use of reversed-phase (RP)-TLC and RP-HPLC techniques, applying octadecyl silica adsorbents and aqueous-organic mobile phases. Organic modifiers: methanol and acetonitrile were used at appropriate concentrations providing optimal retention factors. For both techniques, retention coefficient k in column chromatography and the retardation factor RF expressing retention in TLC were determined. The experimentally measured parameters were presented in a logarithmic scale as log k and RM, respectively. The constructed relationships between log k and RM allowed determining the possibility of TLC for anticipating HPLC retention behavior.

  • M. Dobosz, M. Pitucha, M. Wujec, Acta Pol. Pharm. Drug Res. 53 (1996) 31–38.

    Wujec M. , '' (1996 ) 53 Acta Pol. Pharm. Drug Res. : 31 -38.

  • M. Dobosz, M. Struga, A. Chodkowska, E. Jagiello-Wójtowicz, K. Stepniak, A.E. Koziol, Acta Pol. Pharm. Drug Res. 59 (2002) 281–290.

    Koziol A.E. , '' (2002 ) 59 Acta Pol. Pharm. Drug Res. : 281 -290.

  • K. Walczak, A. Gondela, J. Suwinski, Eur. J. Med. Chem. 39 (2004) 849–853.

    Suwinski J. , '' (2004 ) 39 Eur. J. Med. Chem. : 849 -853.

  • V. Klimešová, L. Zahajská, K. Waisser, J. Kaustová, U. Möllmann, Il Farmaco 59 (2004) 279–288.

    Möllmann U. , '' (2004 ) 59 Il Farmaco : 279 -288.

  • K. Dabak, Ö. Sezer, A. Akar, O. Anaç, Eur. J. Med. Chem. 38 (2003) 215–218.

    Anaç O. , '' (2003 ) 38 Eur. J. Med. Chem. : 215 -218.

  • K. Zamanii, K. Faghihii, T. Tofighii, M.R. Shariatzadeh, Turk. J. Chem. 28 (2004) 95–100.

    Shariatzadeh M.R. , '' (2004 ) 28 Turk. J. Chem. : 95 -100.

  • M. Tatarczak, J. Flieger, H. Szumilo, Chromatographia 61 (2005) 307–309.

    Szumilo H. , '' (2005 ) 61 Chromatographia : 307 -309.

  • T. Tuzimski, Chromatographia 56 (2002) 379–381.

    Tuzimski T. , '' (2002 ) 56 Chromatographia : 379 -381.

  • L.R. Snyder, J.J. Kirkland, Introduction to Modern Liquid Chromatography, Wiley, New York, 1974.

    Kirkland J.J. , '', in Introduction to Modern Liquid Chromatography, Wiley, New York , (1974 ) -.

  • H. Schlitt, F. Geiss, J. Chromatogr. 67 (1972) 261–266.

    Geiss F. , '' (1972 ) 67 J. Chromatogr. : 261 -266.

  • I. Kerimov, G. Ayhan-Kilcigil, B. Can-Eke, N. Altanlar, M. Iscan, J. Enzym. Inhib. Med. Chem. 22 (2007) 696–701.

    Iscan M. , '' (2007 ) 22 J. Enzym. Inhib. Med. Chem. : 696 -701.

  • U. Salgin-Göksen, N. Gökhan-Kelekçi, Ö. Göktas, Y. Köysal, E. Kiliç, S. Isik, G. Aktay, M. Özalp, Bioorg. Med. Chem. 15 (2007) 5738–5751.

    Özalp M. , '' (2007 ) 15 Bioorg. Med. Chem. : 5738 -5751.

  • G. Küçükgüzel, A. Kocatepe, E. De Clercq, F. Sahin, M. Güllüce, Eur. J. Med. Chem. 41 (2006) 353–359.

    Güllüce M. , '' (2006 ) 41 Eur. J. Med. Chem. : 353 -359.

  • O.I. El-Sabbagh, H.M. Rady, Eur. J. Med. Chem. 44 (2009) 3680–3686.

    Rady H.M. , '' (2009 ) 44 Eur. J. Med. Chem. : 3680 -3686.

  • F. Chan, L. Yeung, R. LoBrutto, Y. Kazakevich, J. Chromatogr. A 1082 (2005) 158–164.

    Kazakevich Y. , '' (2005 ) 1082 J. Chromatogr. A : 158 -164.

  • S. Buntz, M. Figus, Z. Liu, Y.V. Kazakevich, J. Chromatogr. A 1240 (2012) 104–112.

    Kazakevich Y.V. , '' (2012 ) 1240 J. Chromatogr. A : 104 -112.

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