JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 28: Issue 3
Development and validation of a high-performance thin-layer chromatographic method for the determination of biomarker β-amyrin in the leaves of different Ficus species
Authors:
Omer A. Basudan College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Perwez Alam College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Nasir A. Siddiqui College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Mohamed F. Alajmi College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Adnan J. Alrehaily College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Saleh I. Alqasoumi College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia

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Maged S. Abdel-Kader College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia

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Prawez Alam College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia

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Abd El Raheim M. Donia College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia

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Pages:
223–228
Online Publication Date:
Jun 2015
Publication Date:
01 Jun 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2015.28.3.5
Keywords:
Ficus species; β-Amyrin; High-performance thin-layer chromatography; Validation
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A simple and sensitive high-performance thin-layer chromatographic (HPTLC) method was developed for the evaluation of biomarker β-amyrin in the leaves of fve different species of genus Ficus (Ficus carica, Ficus nitida, Ficus ingens, Ficus palmata, and Ficus vasta) grown in the Kingdom of Saudi Arabia. Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with solvents toluene–methanol (9:1, v/v) as the mobile phase. After development, the HPTLC plate was derivatized with p-anisalde-hydereagent to give well-resolved and compact spot of β-amyrin. Scanning and quantifcation were done at 550 nm. The system was found to give compact spot for β-amyrin at RF = 0.58. The linear regression analysis data for the calibration plots showed good linear relationship with r2 = 0.998 with respect to area in the concentration range of 100–900 ng. The regression equation for β-amyrin standard was found to be Y = 5.835X + 87. The precisions (n = 6) for β-amyrin were found to be 1.64–1.77% and 1.68–1.84%, respectively, for intra-day and inter-day batches, and the recovery values were found to be 97.6–98.3%. β-Amyrin was found to be present in three species, i.e., F. carica (0.29%, w/w), F. nitida (0. 5 4% w/w), and F. p almata (0.31%, w/w), while it was absent in F. vasta and F. ingens. The statistical analysis proves that the developed method for the quantifcation of β-amyrin is reproducible; hence, it can beemployed for the determination of β-amyrin in plasma and other biological fuids as well as in fnished products avai lable in the market.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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