JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 28: Issue 4
A New Approach to Develop a Standardized Method for Simultaneous Analysis of Astragaloside IV and Formononetin in Radix Astragali by High-Performance Thin-Layer Chromatography
Authors:
Jing Zhang The First Affiliated Hospital of Anhui University of Chinese Medicine, No. 117 Meishanlu, Shushan, Hefei, 230031, China

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Mei Meng The First Affiliated Hospital of Anhui University of Chinese Medicine, No. 117 Meishanlu, Shushan, Hefei, 230031, China

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Ying Jiang The First Affiliated Hospital of Anhui University of Chinese Medicine, No. 117 Meishanlu, Shushan, Hefei, 230031, China

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He Zhang The First Affiliated Hospital of Anhui University of Chinese Medicine, No. 117 Meishanlu, Shushan, Hefei, 230031, China

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Fang Wang The First Affiliated Hospital of Anhui University of Chinese Medicine, No. 117 Meishanlu, Shushan, Hefei, 230031, China

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Pages:
268–273
Online Publication Date:
Aug 2015
Publication Date:
01 Aug 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2015.28.4.1
Keywords:
Radix Astragali; Quality evaluation; Astragaloside IV; Formononetin; High-performance thin-layer chromatography
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A new high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous estimation of astragaloside IV and formononetin in Radix Astragali. Samples were employed to degrease the materials by petroleum ether (boiling point: 60–90°C) and extracted by methanol, and then were alkalized and extracted with n-butanol saturated with water. Separation was achieved on HPTLC plates using petroleum ether (boiling point: 60–90°C) and n-butanol saturated with water-glacial acetic acid as the mobile phase, the results of which were compared with HPLC. The well-resolved peaks for astragaloside IV and formononetin were observed at RF values 0.43 ± 0.02 and 0.75 ± 0.02, respectively. The calibration curves were found linear with a wide range of concentration 1.01–10.10 μg μL−1 with good correlation coefficient for astragaloside IV and formononetin. The method was validated for linearity, precision, reproducibility, accuracy, and limits of detection and quantification. This simple, rapid, sensitive, economic, and reliable HPTLC method is suitable for the routine quantitative analysis and quality control of traditional Chinese medicines (TCMs) such as Radix Astragali, which can be applied for the quality control of saponins and flavonoids in other plants or extracts.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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