JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 28: Issue 4
Development of a High-Performance Thin-Layer Chromatographic Method for Quantification of Cucurbitacin B in Bottle Gourd (Lagenaria siceraria) for Quality Control
Authors:
Bhagyashri Kumbhalkar Agharkar Research Institute, G. G. Agarkar Road, Pune–411 004, India

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Shubhada Tamhankar Agharkar Research Institute, G. G. Agarkar Road, Pune–411 004, India

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Anuradha Upadhye Agharkar Research Institute, G. G. Agarkar Road, Pune–411 004, India

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Pages:
294–299
Online Publication Date:
Aug 2015
Publication Date:
01 Aug 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2015.28.4.5
Keywords:
Cucurbitacin B; Bottle gourd; Accelerated solvent extraction; High-performance thin-layer chromatography–densitometry; ICH guidelines
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Bottle gourd (Lagenaria siceraria (Molina) Standl.) is an important vegetable cucurbit cultivated in the warmer parts of the world. Forms bearing nonbitter fruits are used as vegetables, while those with bitter fruits are used in the Indian Traditional System of Medicine. The presence of cucurbitacins is probably the only distinguishing feature between the two forms. The present communication describes the development and validation of a high-performance thin-layer chromatographic (HPTLC)–densitometric method for the estimation of cucurbitacin B in bottle gourd using the International Conference on Harmonization (ICH) guidelines. The complete methanol extracts obtained by accelerated solvent extraction (ASE) were resolved in the mobile phase chloroform– methanol (9.5:0.5, v/v), wherein cucurbitacin B was resolved at RF 0.67 ± 0.02. Linear relationship was obtained between the values 200 and 1200 ng per spot (r = 0.9909). The limit of detection and limit of quantification were 40 and 200 ng per spot, respectively. The recovery of compound varied between 94 and 96%. Using the developed HPTLC method, the cucurbitacin B content in the vegetative parts (VP) and fruits (F) of the bitter form was found to be 0.017% and 0.062%, respectively, while it was not detected in the nonbitter form. The application of the method to study the accumulation of cucurbitacin B during progressive developmental stages of fruits of both forms revealed an increasing content of cucurbitacin B from 0.001 to 0.085% during the fruit development in the bitter form. In investigations of market samples, cucurbitacin B was detected in two out of six samples indicating admixture of bitter and nonbitter forms. To the best of our knowledge, this study is the first report of the evaluation of bitter and nonbitter forms of bottle gourd for cucurbitacin B using HPTLC. The developed method may be applicable in the routine quality control of marketed products and formulations of bottle gourd.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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