JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 28: Issue 4
Simultaneous Determination of Carbinoxamine, Pholcodine, and Ephedrine in Antitussive Preparation by High-Performance Liquid Chromatography and Thin-Layer Chromatography–Densitometry
Authors:
Azza Aziz Moustafa Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt

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,
Hesham Salem Deraya University, 14511 Cairo, Egypt

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,
Maha Hegazy Cairo University, Kasr-El Aini Street, 11562 Cairo, Egypt

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, and
Omnia A. Mahmoud October University for Modern Sciences and Arts (MSA), 11787 6th October City, Egypt

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Pages:
307–315
Online Publication Date:
Aug 2015
Publication Date:
01 Aug 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2015.28.4.7
Keywords:
Carbinoxamine; Pholcodine; Ephedrine; Reversed-phase high-performance liquid chromatography; Thin-layer chromatography–densitometry
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Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)–densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed on an Inertsil CN-3 column (250 mm × 4.6 mm, 5 μm), using a mobile phase consisting of acetonitrile–distilled water (pH 3.5) using orthophosphoric acid in the ratio 70:30 (v/v) and flow rate of 1.5 mL min−1. Quantitation was achieved with ultraviolet (UV) detection at 220 nm. In method B, TLC analysis was carried out on an aluminum-backed sheet of silica gel 60 F254 layer using chloroform–propanol–ammonia (6:4:0.1, v/v) as the mobile. Quantification was carried out with UV detection at 245 nm. The validation of the proposed methods was applied according to the International Conference on Harmonization (ICH) guidelines. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial dosage form.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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