View More View Less
  • 1 University of Applied Sciences Offenburg, Badstrasse 24, D-77652 Offenburg, Germany
  • | 2 Schwarzwald-Apotheke Offenburg, Hauptstrasse 19, D-77652 Offenburg, Germany
Restricted access

We present a videodensitometric quantification method for methadone in syrup, separated by thin-layer chromatography (TLC). The quantification is based on a derivation reaction with Dragendorff reagent. Measurements were carried out using a 16-bit flatbed scanner. The range of linearity covers two magnitudes of power using the Kubelka-Munk expression for data transformation. The separation method is inexpensive, fast, and reliable.

  • [1]

    R.P. Mattick et al., National Evaluation of Pharmacotherapies for Opioid Dependence (NEPOD): Report of Results and Recommendation, ISBN: 0642824592, ISSN: 1322-5049.

  • [2]

    J. Pothier , N. Galand, P. Maupas, J. Chromatogr. A 1080 (2005) 186191.

  • [3]

    O.M. Berezentseva , I.A. Kluchko, S.I. Nikulicheva, V.S. Padalkina, Sudebno-Meditsinskaia Ekspertiza 38 (1995) 2426.

  • [4]

    A. Scholer , J. Gschwind, E. Hui-Bon-Hoa, N. Grangladen, N. Goepfert, Labor-Med. 14 (1991) 296297.

  • [5]

    A. Koch , Quantitation of methadone (D+L) and l-polamidon in soft drinks, Proceedings of the 9th International Symposium on Instrumental Planar Chromatography, Interlaken, Switzerland 1997, 155159.

    • Search Google Scholar
    • Export Citation
  • [6]

    DAC Anlage J: Halbquantitative Bestimmung mittels DC am Beispiel Methadon-HCl, 2014.

  • [7]

    ICH Harmonised Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology, Q2 (R1), Geneva. http://www.ich.org/LOB/media/MEDIA417.pdf, 2005 (Parent Guideline dated 27 October 1994, Complementary Guideline on Methodology dated 6 November 1996, incorporated in November 2005).

  • [8]

    B. Spangenberg , C.F. Poole, Ch. Weins, Quantitative Thin Layer Chromatography. A Practical Survey, Springer, Heidelberg, 2011.

  • [9]

    B. Spangenberg , J. Planar Chromatogr. 19 (2006) 332341.

  • [10]

    B. Milz , B. Spangenberg, Chromatographia 76 (2013) 13071313.

  • [11]

    B. Spangenberg , Fresenius J. Anal. Chem. 360 (1998) 148151.

  • [12]

    K. Ferenczi-Fodor , B. Renger, Z. Végh, J. Planar Chromatogr. 23 (2010) 173179.

  • [13]

    B. Renger , Z. Vegh, K. Ferenczi-Fodor, J. Chromatogr. A 1218 (2011) 27122721.

  • [14]

    S. Ebel , M. Lorz, M. Weyandt-Spangenberg, Fresenius Z. Anal. Chem. 335 (1989) 960965.

All Time Past Year Past 30 Days
Abstract Views 70 70 2
Full Text Views 19 0 0
PDF Downloads 28 0 0