JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 2
Study of the chromatographic behavior of selected steroid hormones on aluminum oxide plates based on quantitative structure-retention relationships
Authors:
Joanna Nowakowska Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Krzesimir Ciura Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Wiktoria Struck-Lewicka Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Piotr Pikul Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Michał Jan Markuszewski Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Piotr Kawczak Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416, Gdańsk, Poland

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Pages:
113–120
Online Publication Date:
Apr 2016
Publication Date:
01 Apr 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.2.4
Keywords:
Steroid hormones; Quantitative structure-retention relationships; Aluminum oxide; Molecular descriptors
Restricted access

The chromatographic properties of eight steroids: trans-andros-terone, methyltestosterone, testosterone, progesterone, cortisone, hydrocortisone, estrone, and β-estradiol were studied by planar chromatography by use of aluminum oxide plates as well as of four different binary mobile phases (acetonitrile-water, acetonitrile-DMSO, acetone-petroleum ether, and acetone-water). Correlations between chromatographic data and physicochemical parameters were presented as results of established quantitative structure-retention relationships (QSRRs). Principal component analysis (PCA) and multiple linear regression (MLR) showed the possible relationships between the retentions and the physicochemical parameters of the studied compounds. The evaluation of thin-layer chromatographic (TLC) separation power, based on ΔhRF, was also discussed. Finally, two chromatographic systems for the effective separation of glucocorticoids and other analyzed steroid hormones were recommended.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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