JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 2
A validated reversed-phase over-pressured layer chromatography-ultraviolet method for the quantification and optimum recovery of gallic acid in Annona muricata L.
Authors:
Ankita Misra CSIR ‒ National Botanical Research Institute, Lucknow (U.P.) 226001, India

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,
Sharad Srivastava CSIR ‒ National Botanical Research Institute, Lucknow (U.P.) 226001, India

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, and
Ajay Kumar Singh Rawat CSIR ‒ National Botanical Research Institute, Lucknow (U.P.) 226001, India

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Pages:
127–131
Online Publication Date:
Apr 2016
Publication Date:
01 Apr 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.2.6
Keywords:
Gallic acid; Over-pressured layer chromatography; Annona muricata L.; Monophenolic acid
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A validated online over-pressured layer chromatography (OPLC) method was developed for the rapid, cost-effective, and efficient separation of gallic acid. A reversed-phase (RP) ultraviolet (UV)–online OPLC analytical method was developed and validated as per the International Conference on Harmonization (ICH) guidelines. Methanolic extract of Annona muricata (fruit) was prepared by cold maceration. Separation of marker was achieved on RP-OPLC plates (5 × 20 cm) with isocratic solvent system of 0.1% acetic acid, water and methanol (30:70) at a flow rate of 0.15 mL min−1; detection was carried out at λmax 270 nm. The base peak of standard gallic acid was at 5.441 min with good linearity (r2 > 0.999), precision, and accuracy. The limit of detection (LOD) (521.84 mg mL−1) and limit of quantification (LOQ) (1581.336 mg mL−1) values reflect that the method is sensitive with high peak purity; the recoveries of analyte were 99 to 103%. The achievement of the method was the early retention time of gallic acid which in turn increased the efficiency of the quantification of the targeted marker in a short duration of time for even larger number of samples in plant extract as well as biological fluids for pharmacokinetic studies. The application of the method can be extended in regulatory guidelines for the quality control of herbal drugs/products and formulations. The method is rapid and economical in terms of solvent consumption and, hence, can be preferred over other high-performance thin-layer chromatographic or liquid chromatographic methods.

  • [1]

    M. Karamac , A. Kosinska, R.B. Pegg, Polish J. Food Nutri. Sci. 15 (2006) 55–58.

  • [2]

    I. Kubo , N. Masuoka, T. Joung, K. Shimizu, K. Nihei, Open Bioact. Compd. J. 3 (2010) 1–11.

  • [3]

    Z. Sun , L. Zhao, L. Zuo, C. Qi, P. Zhao, X. Hou, J. Chromatogr. B 858 (2014) 55–62.

  • [4]

    F. Maa , X. Gong, X. Zhou, Y. Zhao, M. Li, J. Ethnopharmacol. 162 (2015) 377–383.

  • [5]

    W. Phakthong , B. Liawruangrath, S. Liawruangrath, Talanta 130 (2014) 577–584.

  • [6]

    E. Tyihak , E. Mincsovics, A.M. Moricz, J. Chromatogr. A 1232 (2012) 3–18.

  • [7]

    C.J.M. Cardozo , V.V. Lozano, D.P.Y. Betancur, H.J.C. Velasquez, J.R.C. Valenzuela, Revista Facultad Nacional de Agronomi Medellin 65 (2012) 6477–6486.

    • Search Google Scholar
    • Export Citation
  • [8]

    V.C. George , D.R.N. Kumar, V. Rajkumar, P.K. Suresh, R.A. Kumar, Asian Pacific J. Cancer Prevent. 13 (2011) 699–704.

  • [9]

    D.K. Singh , S.K. Srivastava, A.K.S. Rawat, Acta Chromatogr. 19 (2007) 246–252.

  • [10]

    N.A. Santagati , L. Salerno, G. Attaguile, F. Savoca, G. Ronsisvalle, J. Chromatogr. Sci. 46 (2008) 150–156.

  • [11]

    A. Zhang , L. Wan, C. Wu, Y. Fang, G. Han, H. Li, Z. Zhang, H. Wang, Molecules 18 (2013) 14241–14257.

  • [12]

    ICH, Q2R1 Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization, Geneva, 2005.

  • [13]

    P. Oroszlan , G. Verzar-Petri, E. Mincsovics, T. Székely, A. Vastagh, Proc. Intern. Symposium on TLC with Special Emphasis on OPLC (Szeged, 1984), Labor MIM, Budapest (E. Tyihák ed.), 1986, pp. 343–352.

    • Search Google Scholar
    • Export Citation
  • [14]

    M. Hevesi , A.M. Moricz, Z.K. Veghely, M. Toth, Gy. Katay, E. Tyihak, J. Planar Chromatogr. 23 (2010) 411–414.

  • [15]

    A.M. Moricz , S. Szarka, P.G. Ott, E.B. Hethelyi, E. Szoke, E. Tyihak, Med. Chem. 8 (2012) 85–94.

  • [16]

    S. Srivastava , A. Misra, P.K. Shukla, B. Kumar, S. Lata, A.K.S. Rawat, RSC Adv. 4 (2014) 60902–60906.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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