JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 3
Development and validation of a high-performance thin-layer chromatographic—densitometric method for the quantification of apigenin in Ocimum basilicum L. seed (Takhmaria)
Authors:
Nisha H. Parikh Nirma University, Ahmedabad, Gujarat, India
Arihant School of Pharmacy and Bio-Research Institute, Adalaj, Gandhinagar, Gujarat, India

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and
Charmy S. Kothari Nirma University, Ahmedabad, Gujarat, India

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Pages:
216–220
Online Publication Date:
Jun 2016
Publication Date:
01 Jun 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.3.8
Keywords:
Apigenin; Ocimum basilicum seed (Takhmaria); High-performance thin-layer chromatography; Densitometric determination; Methanolic extract
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A simple, selective, precise, and accurate high-performance thin-layer chromatographic (HPTLC) method has been developed for the quantification of Ocimum basilicum seeds for their apegenin content. Densitometric evaluation was carried out on precoated silica gel G60 F254 HPTLC plates using toluene—acetone—formic acid (5:4:1, v/v) as the mobile phase. Scanning and densitometric evaluations were done at 340 nm. The calibration curve was plotted in the concentration range of 100–600 ng band−1, and the method was found linear with r2 = 0.995. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines for accuracy, precision, range, linearity, limit of detection (LOD), and limit of quantification (LOQ). The validated HPTLC method was used for the estimation of apigenin in O. basilicum seeds. The developed method can be applicable for the identification and quantification of apigenin in complex mixtures of phytochemical and marker-based standardization of plant samples as well as extracts containing O. basilicum seeds.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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