JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 4
Thin-layer chromatography and chemometric analysis in the fingerprinting of selected Scutellaria species
Authors:
Mirosław A. Hawrył Medical University of Lublin, 4a, Chodźki St., 20-093 Lublin, Poland

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Anna Hawrył Medical University of Lublin, 4a, Chodźki St., 20-093 Lublin, Poland

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Ryszard Świeboda Medical University of Lublin, 4a, Chodźki St., 20-093 Lublin, Poland

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Małgorzata Niemiec Medical University of Lublin, 4a, Chodźki St., 20-093 Lublin, Poland

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Monika Waksmundzka-Hajnos Medical University of Lublin, 4a, Chodźki St., 20-093 Lublin, Poland

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Pages:
256–263
Online Publication Date:
Aug 2016
Publication Date:
01 Aug 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.4.3
Keywords:
Scutellaria ; Flavonoids; Phenolic acids; Thin-layer chromatography; Fingerprint; Chemometric; Principal component analysis
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Seven different Scutellaria species were analyzed using the extraction procedure (Soxhlet apparatus, dichloromethane, and methanol as solvents) and thin-layer chromatography method. Selected standards of flavonoids and phenolic acids (caffeic acid, chlorogenic acid, ferulic acid, baicalein, wogonin, baicalin, chrysin, quercetin, scutellarin, hesperetin, hesperidin, apigenin, luteolin, rutin, and kaempferol) were separated using silica gel thin-layer chromatography (TLC) plates with the mobile phase consisting of ethyl acetate—toluene—formic acid (5:4.9:0.1, v/v) for dichloromethane and methanolic extracts. Dichloromethane extracts were also developed using cyanopropyl-bonded silica gel with the following mobile phases: propan-2-ol—n-heptane—formic acid (5:4.9:0.1, v/v) and methanol—water—formic acid (6:3.9:0.1, v/v), and after drying, they were sprayed using the anisaldehyde reagent. In the case of methanolic extracts, the same non-aqueous eluent was used and the aqueous eluent consisting of methanol—water—formic acid (4:5.9:0.1, v/v). The presence of selected standards in Scutellaria species was confirmed. The similarities between the obtained fingerprint chromatograms were performed using chemometric methods, the similarity coefficients (Pearson’s correlation coefficient, determination coefficient, and congruence coefficient), distance indices (Euclidean distance, Manhattan distance, and Chebyshev’s distance), and multi-scale structural similarity (MS-SSIM).

  • [1]

    X. Shang , X. He, X. He, M. Li, R. Zhang, P. Fan, Q. Zhang, Z. Jia, J. Ethnopharmacol. 24 (2010) 279–313.

  • [2]

    I. Grzegorczyk-Karolak , Ł. Kuźma, H. Wysokińska, Acta Physiol. Plant. 37 (2015) 1736. DOI: 10.1007/s11738-014-1736-0.

  • [3]

    K. Yu , Y. Gong, Z. Lin, Y. Cheng, J. Pharm. Biomed. Anal. 43 (2007) 540–548.

  • [4]

    I. Grzegorczyk-Karolak , Ł. Kuźma, H. Wysokińska, J. Med. Plants Res. 7 (2013) 3003–3313.

  • [5]

    J. Pan , C.S. Yuan, C.J. Lin, Z.J. Jia, R.L. Zheng, Pharmazie 58 (2003) 767–775.

  • [6]

    I. Grzegorczyk-Karolak , Ł. Kuźma, H. Wysokińska, Acta Physiol. Plant. 38 (2016) 7. DOI: 10.1007/s11738-015-2024-3.

  • [7]

    R. Pan , F. Guo, H. Lu, W. Feng, Y.Z. Liang, J. Pharm. Biomed. Anal. 55 (2011) 391–396.

  • [8]

    C. Gousiadou , A. Karioti, J. Heilmann, H. Skaltsa, Phytochemistry 68 (2007) 1799–1804.

  • [9]

    J.C. Anderson , W.M. Blaney, M.D. Cole, L.L. Fellows, S.V. Ley, R.N. Sheppard, M.S.J. Simmonds, Tetrahedron Lett. 30 (1989) 4737–4740.

  • [10]

    M. Bruno , N. Vassallo, M.S.J. Simmonds, Phytochemistry 50 (1999) 973–976.

  • [11]

    M.S. Mohammed , M.F. Alajmi, P. Alam, H.S. Khalid, A.M. Mahmoud, W.J. Ahmed, Asian Pac. J. Trop. Biomed. 4 (2014) 203–208.

  • [12]

    A.G. Patil , S.P. Koli, D.A. Patil, J. Pharm. Res. 6 (2013) 145–150.

  • [13]

    S. Srinivasan , W. Wankhar, S. Rathinasamy, R. Rajan, J. Pharm. Anal. 6 (2016) 125‒131.

  • [14]

    M.F. Alajmi , P. Alam, Asian Pac. J. Trop. Biomed. 3 (2013) 341–347.

  • [15]

    A. Bansal , V. Chhabra, R.K. Rawal, S. Sharman, J. Pharm. Anal. 4 (2014) 223–233.

  • [16

    Ł. Komsta , Ł. Cieśla, A. Bogucka-Kocka, A. Józefczyk, J. Kryszeń, M. Waksmundzka-Hajnos, J. Chromatogr. A 1218 (2011) 2820–2825.

  • [17]

    M. Olech , Ł. Komsta, R. Nowak, Ł. Cieśla, M. Waksmundzka-Hajnos, Food Chem. 132 (2012) 549–553.

  • [18]

    D. Rouse , S. Hemami, Analyzing the Role of Visual Structure in the Recognition of Natural Image Content with Multi-Scale SSIM; Proceedings of SPIE, 6806, Human Vision and Electronic Imaging XIII, San Jose, CA, 2008.

    • Search Google Scholar
    • Export Citation
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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