JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 5
Quantitative analysis of phytochemicals from Inula cappa roots
Authors:
Jyoti Kalola L. M. College of Pharmacy, Navarangpura, Ahmedabad, 380 009, Gujarat, India

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Rahul Shah L. J. Institute of Pharmacy, Sarkhej, Ahmedabad, 382 210, Gujarat, India

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Mamta Shah L. M. College of Pharmacy, Navarangpura, Ahmedabad, 380 009, Gujarat, India

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Pages:
336–340
Online Publication Date:
Oct 2016
Publication Date:
01 Oct 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.5.2
Keywords:
Inula cappa ; High-performance thin-layer chromatography; Quantitative analysis
Restricted access

Inula cappa (family Compositae) is used in the Ayurvedic medicinal system for the treatment of bronchitis, diabetes, fever, hypertension, and rheumatism. The proposed high-performance thin-layer chromatography (HPTLC) study offers coherent evaluation of isoalantolactone, germacranolide, β-sitosterol, and lupeol from I. cappa root. Methanolic solutions of isoalantolactone, germacranolide, β-sitosterol, and lupeol were applied on an HPTLC plate and they were scanned at 525 nm. The mobile phase toluene—methanol (9.4:0.6, v/v) was used for all the phytochemicals. After development, all the plates were air-dried at room temperature, derivatized with anisaldehyde–sulfuric acid reagent and heated at 105°C. This study aids the identification of these compounds and provides an easy and simple method for the simultaneous estimation of these markers in the I. cappa roots. The method would serve as an expedient tool in routine analyses to corroborate the drug through good constancy.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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