Authors:
Shikhar Verma CSIR — National Botanical Research Institute, Lucknow — 226 001, India
Amity University, Lucknow Campus, Uttar Pradesh, India

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Abhishek Gupta CSIR — National Botanical Research Institute, Lucknow — 226 001, India

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Malipeddi Venkata Ramana Amity University, Lucknow Campus, Uttar Pradesh, India

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Ajay Kumar Singh Rawat CSIR — National Botanical Research Institute, Lucknow — 226 001, India

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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of gallic acid, vanillic acid, protocatechuic acid, and quercetin in methanolic fractions of Limonia acidissima L. fruits was developed for the first time for this species. Methanol was found to be the best for the highest possible recovery of the target analytes. For achieving good separation, a mobile phase of toluene–ethyl acetate–formic acid (5:4:1 v/v) was used. The densitometric determination was carried out at 310 and 254 nm in reflection–absorption mode. The calibration curves were linear in the range of 100–600 ng per spot for gallic acid, vanillic acid, protocatechuic acid, and quercetin. The methanolic fractions of L. acidissima L. fruits showed the presence of gallic acid (0.07%), vanillic acid (0.16%), protocatechuic acid (0.06%), and quercetin (0.14%). The proposed method is simple, precise, specific, and accurate. The statistical analysis of the data obtained proves that the method is reproducible and selective and can be used for the routine analysis of the reported phenolic compounds in crude drug and extracts. The simultaneous quantification of these compounds has not been reported yet in L. acidissima L., which may be utilized for the proper standardization of the drug.

  • [1]

    S. Ponnuraj , D. Jaganathan, M. Kanagarajan, T. P. Deivamarudachalam, Int. J. Pharm. Pharm. Sci. 7 (2015) 383389.

  • [2]

    P. Vijayvargia , R. Vijayvergia, Int. J. Pharm. Sci. Rev. Res. 28 (2014) 191195.

  • [3]

    K. R. Kirtikar , B. D. Basu, Indian Medicinal Plants, Vol. 1, International Book Distributors, Dehradun, 2005, pp. 478479.

  • [4]

    K. Llango , V. Chitra, Trop. J. Phys. Res. 37 (2010) 120125.

  • [5]

    K. Sunitha , G. K. Mohan, RJPBCS 4 (2013) 439444.

  • [6]

    S. Pandey , G. Satpathy, R. K. Gupta, J. Pharmacogn. Phytochem. 3 (2014) 8188.

  • [7]

    A. Thomas , N. R. Ponnammal, Ancient Sci. Life 25 (2005) 5761.

  • [8]

    K. Llango , V. Chitra, Pharm. Lett. 1 (2009) 117125.

  • [9]

    M. Jain , A. Trivedi, S. H. Mishra, Am. J. Plant Sci. 1 (2010) 1216.

  • [10]

    ICH, Q2A, Text on Validation of Analytical Procedures, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1994.

  • [11]

    ICH, Q2B, Validation of Analytical Procedures: Methodology, Harmonized Tripartite Guideline prepared within the International Conference on Harmonization of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Geneva, 1996.

  • [12]

    A. Gupta , S. Verma, S. Khatoon, H. Dwivedi, A. K. S. Rawat, J. Planar Chromatogr. 28 (2015) 452457.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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