JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 29: Issue 6
High-performance thin-layer chromatographic analysis for the simultaneous quantification of lupeol and ursolic acid in the methanolic fraction of four different species of Bauhinia
Authors:
Abhishek Gupta CSIR-National Botanical Research Institute, Lucknow, India
Babu Banarsi Das University, Lucknow, India

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,
Shikhar Verma CSIR-National Botanical Research Institute, Lucknow, India

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,
Harinath Dwivedi Babu Banarsi Das University, Lucknow, India

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, and
Ajay Kumar Singh Rawat CSIR-National Botanical Research Institute, Lucknow, India

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Pages:
423–428
Online Publication Date:
Dec 2016
Publication Date:
01 Dec 2016
Article Category:
Research Article
DOI:
https://doi.org/10.1556/1006.2016.29.6.4
Keywords:
Bauhinia ; High-performance thin-layer chromatography; Lupeol; Ursolic acid
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A high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantitative determination of lupeol and ursolic acid in the methanolic fraction of four different species of Bauhinia leaves was developed for the first time. For achieving good separation, a mobile phase of toluene—ethyl acetate—formic acid (8:2:0.1, v/v) was used. The densitometric determination was carried out at 550 nm and 520 nm in reflection—absorption mode for lupeol and ursolic acid, respectively, which were linear in the range of 100–600 ng per band. During the analysis, lupeol (0.15%) and ursolic acid (0.11%) were found to be the highest in the leaves of B. acuminata. The proposed method is simple, precise, specific, and accurate. The statistical analysis of the obtained data proves that the method is reproducible and selective and can be used for the routine analysis of the reported terpenoids in crude drug and extracts. The simultaneous quantification of these compounds has not yet been reported in the leaves of the studied Bauhinia species which may be utilized for the proper standardization of these species.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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