View More View Less
  • 1 Beni-Suef University, Beni-Suef, 62514, Egypt
  • | 2 Nahda University, Beni-Suef, 62514, Egypt
Restricted access

The presented study was intended to design two validated, simple, and precise chromatographic methods for the determination of chlorzoxazone (CHZ) and diclofenac potassium (DIC) in the presence of chlorzoxazone nephrotoxic degradation product, 2-amino-4-chlorophenol (ACP) which was reported to be its main impurity. Reversed-phase high-performance liquid chromatography (RP-HPLC) was the first method where chromatographic separation was performed on ZORBAX Eclipse Plus C8 column using methanol—water—phosphoric acid (75:25:0.05, by volume) as the mobile phase at a flow rate of 1 mLmin−1. CHZ, DIC, and ACP retention times were found to be 4.26, 7.94, and 3.17, respectively, using photodiode array detector (DAD) at 230 nm. The calibration curves showed good linear relationships in the concentration ranges of 3–45 μg mL−1 for CHZ, 3–40 μg mL−1 for DIC, and 5–45 μg mL−1 for ACP. The second method was thin-layer chromatography (TLC) at which chromatographic separation was carried out on Merck TLC silica gel 60 F254 aluminum plates followed by measurement of separated bands at 230 nm and using chloroform—ethanol—triethylamine (9:1:0.1, by volume) as the developing system. The studied components were successfully separated with significantly different Rf values (CHZ, Rf = 0.63; DIC, Rf = 0.35; ACP, Rf = 0.42). Linearity was constructed in the range of 1.2–5 μg band−1 for CHZ, 0.5–4 μg band−1 for DIC, and 0.4–4 μg band−1 for ACP. The developed methods were applied to Declophen plus® capsules, and no interference from excipients was observed. The methods were validated as per the United States Pharmacopeia (USP) guidelines, and they were compared favorably with the reported method.

  • [1]

    The United States Pharmacopeia, 32nd edn., National Formulary 27, United States Pharmacopeial Convention, Rockville, MD, 2009.

  • [2]

    M. M. Ellaithy , N. A. El-Regehy, M. A. El-Ghobashy, Il Farmaco 58 (2003) 337342.

  • [3]

    E. A. Abdelaleem , N. S. Abdelwahab, J. Chromatogr. Sci. 51 (2013) 187191.

  • [4]

    M. A. Badgujar, S. G. Pingale, K. V. Mangaonkar, E-J. Chem. 8 (2011) 12061211.

  • [5]

    R. Panchumarthy , D. R. Garikapati, A. Akaula, S. B. Puttagunta, J. Chem. Pharm. Sci. 6 (2013) 181188.

  • [6]

    J. T. Stewart , Anal. Profiles Drug Subst. Excipients 16 (1987) 119144.

  • [7]

    British Pharmacopoeia, Vol. I, Stationery Office, Medicines and Healthcare Products Regulatory Agency, London, 2003, pp. 605–606.

  • [8]

    R. A. Patel , S. H. Joshi, World J. Pharm. Pharm. 3 (2014) 12791291.

  • [9]

    Sigma-Aldrich Catalogue; www.sigmaaldrich.com; accessed on 16 April, 2016.

  • [10]

    Hazardous Chemicals Fact Sheet, New Jersey Department of Health and Senior Services, Trenton, NJ, 1996.

  • [11]

    F. F. Belal , M. K. Sharaf El-Din, F. A. Aly, M. I. El-Awady, Int. J. Pharma. Sci. Res. 2 (2011).

  • [12]

    S. A. Patel , K. M. Prajapati, Int. J. ChemTech Res. 5 (2013) 20352040.

  • [13]

    K. M. Prajapati , Int. J. Pharmamedix India 1 (2013) 258269.

  • [14]

    S. A. Patel , K. M. Prajapati, Pharmatutor (2013) 19; (http://www. pharmatutor. org/articles/development-and-validation-rp-hplcmethod- for-simultaneous-estimation-of-chlorzoxazone-diclofenac- sodium-in-combination).

    • Search Google Scholar
    • Export Citation
  • [15]

    M. S. Bhatia , S. G. Kaskhedikar, S. C. Chaturvedi, Indian Drugs 34 (1997) 149153.

  • [16]

    S. Ravisankar , M. Vasudevan, M. J. Nanjan, Bijukurian, B. Suresh, Indian Drugs 34 (1997) 663665.

  • [17]

    J. L. Chawla , R. A. Sodhi, R. T. Sane, Indian Drugs 33 (1996) 171178.

  • [18]

    A. Goyal , S. Jain, Acta Pharm. Sci. 49 (2007) 147151.

  • [19]

    M. B. Kulkarni , P. B. Dange, S. G. Walode, J. Pharm. Sin. 3 (2012) 643652.

  • [20]

    S. Pawar , A. Kale, M. Amrutkar, A. Bhosale, Asian J. Res. Chem. 2 (2009) 306308.

  • [21]

    R. Ganesh , U. J. Dhorda, M. Sundaresan, Indian Drugs 38 (2001) 483486.

  • [22]

    J. Patel , P. Patel, Int. J. Pharm. Pharm. Sci. 6 (2014) 632637.

  • [23]

    The United States Pharmacopeia, National Formulary 35, United States Pharmacopeia Convention Inc., 30th edn., Rockville, MD, 2012.

  • [24]

    M. M. Srivastava , High P erformance Thin L ayer Chromatography (HPTLC), Springer, Heidelberg, Dordrecht, London, 2011, p. 3338.

  • [25]

    The United States Pharmacopeia “National Formulary USP”; The Official Compendia of Standards, USP 34-NF 29, Rockville, MD, 2011.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
May 2021 9 0 0
Jun 2021 9 0 0
Jul 2021 3 0 0
Aug 2021 8 0 0
Sep 2021 7 0 0
Oct 2021 2 0 0
Nov 2021 0 0 0