View More View Less
  • 1 Medical University of Lublin, 4a, Chodźki Street, 20-093 Lublin, Poland
  • 2 Medical University of Lublin, 1, Chodźki Street, 20-093 Lublin, Poland
Restricted access

Satisfactory separation of a test mixture of 19 dyes with a general elution problem was obtained by reversed-phase stepwise gradient thin-layer chromatography with single void volume of the mobile phase. An effect of the supplementary mobile phase flux to the surface of the adsorbent layer, observed previously, was eliminated. This improves the flow profile of the mobile phase and the shape of spots, and increases the repeatability of the results within the same plate and between studies. The experimental values of the relative zone/spot position, Rpg, showed good correlation with the results predicted by the computing calculation.

  • [1]

    J. Sherma , Anal. Chem. 82 (2010) 48954910.

  • [2]

    C. Tistaert , B. Dejaegher, Y. Vander Heyden, Anal. Chim. Acta 690 (2011) 148161.

  • [3]

    K. Misra , R. Tulsawani, R. Shyam, D. K. Meena, G. Morlock, J. Liq. Chromatogr. Relat. Technol. 35 (2012) 13641387.

  • [4]

    T. Piech , B. Majer-Dziedzic, A. Kostruba, E. M. Grzelak, I. M. Choma, J. Liq. Chromatogr. Relat. Technol. 39 (2016) 292297.

  • [5]

    R. M. Elsanhoty , M. F. Ramadan, Food Control 22 (2016) 155161.

  • [6]

    M. Jaskiewicz , M. Orlowska, G. Olizarowicz, D. Migon, D. Grzywacz, W. Kamysz, Int. J. Pept. Res. Ther. 22 (2016) 155161.

  • [7]

    C. Oellig , T. Melde, J. Chromatogr. A 1441 (2016) 126133.

  • [8]

    M. Balouiri , M. Sadiki, S. K. Ibnsouda, J. Pharm. Anal. 6 (2016) 7179.

  • [9]

    W. Jesionek , A. M. Móricz, P. G. Ott, B. Kocsis, G. Horváth, I. M. Choma, J. AOAC Int. 98 (2015) 857861.

  • [10]

    L. Ciesla , Med. Chem. 8 (2012) 102111.

  • [11]

    S. Görög , J. Pharm. Biomed. Anal. 69 (2012) 28.

  • [12]

    P. Bernard-Savary , C. F. Poole, J. Chromatogr. A 1421 (2015) 184202.

  • [13]

    G. E. Morlock , J. Chromatogr. A 1382 (2015) 8796.

  • [14]

    S. Gocan , G. Cimpan, J. Liq. Chromatogr. Relat. Technol. 27 (2004) 13771411.

  • [15]

    M. Waksmundzka-Hajnos , J. Sherma, T. Kowalska (eds.), Thin Layer Chromatography in Phytochemistry, CRC Press, Taylor & Francis Group, Boca Raton, FL, 2008.

    • Search Google Scholar
    • Export Citation
  • [16]

    W. Golkiewicz , in: J. Sherma and B. Fried (eds.), Handbook of Thin-Layer Chromatography, Marcel Dekker, Inc. , New York, NY, 2003.

  • [17]

    C. Liteanau , S. Gocan, Gradient Liquid Chromatography, John Wiley & Sons Inc. , Chichester, 1974.

  • [18]

    T. H. Dzido , in: Sz. Nyiredy (ed.), Planar Chromatography –A Retrospective View for the Third Millennium, Springer, Budapest, 2001.

  • [19]

    A. Niederwieser , M. Brenner, Experientia 21 (1965) 105109.

  • [20]

    A. Niederwieser , C. C. Honegger, Adv. Chromatogr. 2 (1966) 123167.

  • [21]

    F. Geiss , H. Schlitt, Chromatographia 1 (1968) 392402.

  • [22]

    M. Brenner , A. Niederwieser, Experientia 17 (1961) 237238.

  • [23]

    E. Soczewinski , G. Matysik, J. Liq. Chromatogr. Relat. Technol. 8 (1985) 11251238.

  • [24]

    E. Soczewinski , J. Chromatogr. 369 (1986) 1117.

  • [25]

    E. Soczewinski , W. Markowski, J. Chromatogr. 370 (1986) 6373.

  • [26]

    G. Matysik , E. Soczewinski, J. Planar Chromatogr. 9 (1996) 404412.

  • [27]

    G. Matysik , E. Soczewinski, J. Chromatogr. 446 (1988) 275282.

  • [28]

    G. Matysik , E. Soczewinski, Chromatographia 26 (1988) 178180.

  • [29]

    T. H. Dzido , E. Soczewinski, J. Chromatogr. 516 (1990) 461466.

  • [30]

    W. Golkiewicz , M. Jaroniec, J. H igh R es. C hromatogr. C hromatogr. Commun. 1 (1978) 245249.

  • [31]

    W. Markowski , J. Chromatogr. 485 (1989) 517532.

  • [32]

    W. Markowski , J. Chromatogr. 635 (1993) 283289.

  • [33]

    W. Markowski , E. Soczewinski, J. Chromatogr. 623 (1992) 139147.

  • [34]

    T. H. Dzido , J. Planar Chromatogr. 6 (1993) 378381.

  • [35]

    W. Markowski , E. Soczewinski, Chromatographia 36 (1993) 330336.

  • [36]

    R. Cela , M. Lores, Comput. Chem. 20 (1996) 175191.

  • [37]

    K. Burger , Z. Fresenius, Anal. Chem. 318 (1984) 228233.

  • [38]

    W. Markowski , K. Wróblewski, T. H. Dzido, J. Planar Chromatogr. 25 (2012) 200207.

  • [39]

    A. Halka-Grysinska , T. H. Dzido, E. Sitarczyk, A. Klimek-Turek, A. Chomicki, J. Liq. Chromatogr. Relat. Technol. 39 (2016) 257263.

  • [40]

    G. Vázquez , E. Alvarez, J. M. Navaza, J. Chem. Eng. Data 40 (1995) 611614.

  • [41]

    T. H. Dzido , R. Majewski, B. Polak, W. Golkiewicz, E. Soczewinski, J. Planar Chromatogr. 16 (2003) 176182.

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jun 2020 0 0 0
Jul 2020 1 0 0
Aug 2020 5 0 0
Sep 2020 1 0 0
Oct 2020 0 0 0
Nov 2020 1 0 0
Dec 2020 0 0 0